Cell-laden microengineered gelatin methacrylate hydrogels.
Cell-laden microengineered gelatin methacrylate hydrogels.
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DOI：
10.1016/j.biomaterials.2010.03.064
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发表时间：
2010-07
期刊：
影响因子：
14
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
细胞微环境在改善微工程组织的功能中起着不可或缺的作用。在工程组织中的微结构控制可以通过细胞负载水凝胶的光模式来实现。然而，尽管光聚合水凝胶的模式保真度很高，但许多此类材料没有细胞响应性，并且生物降解性有限。在这里，我们展示了甲基丙烯酸明胶（GelMA）作为一种廉价的，细胞响应的水凝胶平台，用于创建细胞负载的微组织和微流体装置。无论将细胞植入微图案的GelMA底物上，还是包裹在微制造的GelMA水凝胶中，细胞都很容易结合、增殖、伸长和迁移。通过对甲基丙烯酸化程度和凝胶浓度的改性，GelMA的水化和机械性能可调，适用于各种应用。GelMA的图案保真度和分辨率高，可用于制造可渗透的微流控通道。此外，GelMA微模式可用于创建细胞微模式，用于体外细胞研究或3D显微组织制造。这些数据表明，GelMA水凝胶可用于创建复杂的细胞反应性微组织，如内皮化微血管，或用于其他需要细胞反应性微工程水凝胶的应用。
The cellular microenvironment plays an integral role in improving the function of microengineered tissues. Control of the microarchitecture in engineered tissues can be achieved through photopatterning of cell-laden hydrogels. However, despite high pattern fidelity of photopolymerizable hydrogels, many such materials are not cell-responsive and have limited biodegradability. Here we demonstrate gelatin methacrylate (GelMA) as an inexpensive, cell-responsive hydrogel platform for creating cell-laden microtissues and microfluidic devices. Cells readily bound to, proliferated, elongated and migrated both when seeded on micropatterned GelMA substrates as well as when encapsulated in microfabricated GelMA hydrogels. The hydration and mechanical properties of GelMA were demonstrated to be tunable for various applications through modification to the methacrylation degree and gel concentration. Pattern fidelity and resolution of GelMA was high and it could be patterned to create perfusable microfluidic channels. Furthermore, GelMA micropatterns could be used to create cellular micropatterns for in vitro cell studies or 3D microtissue fabrication. These data suggest that GelMA hydrogels could be useful for creating complex, cell-responsive microtissues, such as endothelialized microvasculature, or for other applications that requires cell-responsive microengineered hydrogels.
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影响因子：
14
作者：
Benton, Julie A.;Fairbanks, Benjamin D.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
DOI：
10.1016/0304-4165(80)90335-9
发表时间：
1980-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BARKER, H;OLIVER, R;STEPHEN, L
通讯作者：
STEPHEN, L
影响因子：
--
作者：
Benton JA;DeForest CA;Vivekanandan V;Anseth KS
通讯作者：
Anseth KS
影响因子：
4.9
作者：
Khademhosseini, Ali;Eng, George;Burdick, Jason A.
通讯作者：
Burdick, Jason A.