Relation of depot-specific adipose inflammation to insulin resistance in human obesity.
Relation of depot-specific adipose inflammation to insulin resistance in human obesity.
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DOI：
10.1038/nutd.2012.3
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发表时间：
2012-03-05
影响因子：
6.1
通讯作者：
Gokce, N.
中科院分区：
文献类型：
--
作者：
Bigornia, S. J.;Farb, M. G.;Mott, M. M.;Hess, D. T.;Carmine, B.;Fiscale, A.;Joseph, L.;Apovian, C. M.;Gokce, N.
与肥胖相关的脂肪组织炎症的低度状态与全身代谢功能障碍的机制有关。然而，临床表型与储库特异性炎症的关系尚未在人类肥胖中得到很好的研究。表征皮下和内脏脂肪库的炎症状态，通过组织中存在的巨噬细胞冠状结构（CLS）作为慢性炎症的标志进行评估，并确定全身胰岛素抵抗与皮下和内脏脂肪中炎症异常的关系。我们在计划的减肥手术中同时从92名肥胖参与者（年龄42±11岁; BMI ≤ 30 kg m−2）的皮下和内脏（网膜和肠系膜）仓库收集脂肪组织。使用免疫组织化学，我们根据皮下（CLS）、网膜（CLSo）和肠系膜（CLSm）脂肪库中巨噬细胞CLS的存在与否，分别将个体归类为CLS+或CLS-。大多数参与者表现出脂肪组织炎症，表现为皮下和腹内内脏贮库中存在CLS（CLS+）。皮下脂肪中的CLS状态对于内脏脂肪的炎症是高度敏感和适度特异的。在多变量模型中，血浆胰岛素和稳态模型评估水平与CLS+状态呈正相关，与年龄、腰围、BMI和2型糖尿病无关，并随着脂肪区域数量的增加而恶化。在严重肥胖的参与者中，全身性胰岛素抵抗与皮下和内脏脂肪库的脂肪炎症有关。研究结果表明，经皮活检更容易进入的皮下区域的检查可能在临床研究中被证明是有用的，这些研究旨在调查脂肪表型与人类疾病的关系。
A low-grade state of adipose tissue inflammation associated with obesity has been linked to mechanisms of systemic metabolic dysfunction. However, the relation of clinical phenotypes to depot-specific inflammation has not been well examined in human obesity. To characterize the inflammatory status of subcutaneous and visceral fat depots, as assessed by tissue presence of macrophage crown-like structures (CLS) as a hallmark of chronic inflammation, and determine the relation of systemic insulin resistance to inflammatory abnormalities in subcutaneous and visceral fat. We collected adipose tissue simultaneously from subcutaneous and visceral (omental and mesenteric) depots in 92 obese participants (age 42±11 years; BMI⩾30 kg m−2) during planned bariatric surgery. Using immunohistochemistry, we categorized individuals as CLS+ or CLS− based on the presence or absence, respectively, of macrophage CLS in subcutaneous (CLSs), omental (CLSo) and mesenteric (CLSm) adipose depots. The majority of participants exhibited adipose tissue inflammation manifest by the presence of CLS (CLS+) in both subcutaneous and intra-abdominal visceral depots. CLS status in subcutaneous fat was highly sensitive and modestly specific for inflammation of visceral fat. In multivariable models, plasma insulin and homeostatis model assessment levels were positively associated with CLS+ status in all depots independent of age, waist circumference, BMI and type 2 diabetes, and worsened with the increasing number of adipose regions involved. In severely obese participants, systemic insulin resistance is linked to adipose inflammation in both subcutaneous and visceral depots. The findings suggest that examination of subcutaneous regions that are more easily accessible by transcutaneous biopsy may prove useful in clinical studies designed to investigate adipose phenotypes in relation to human disease.
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影响因子：
120.7
作者：
Flegal, Katherine M.;Carroll, Margaret D.;Curtin, Lester R.
通讯作者：
Curtin, Lester R.
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作者：
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通讯作者：
Jialal, Ishwarlal
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5.8
作者：
Aron-Wisnewsky, Judith;Tordjman, Joan;Clement, Karine
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