Characterization and expression of the mouse endonuclease G gene.
Characterization and expression of the mouse endonuclease G gene.
复制标题
小鼠核酸内切酶 G 基因的表征和表达。
作者：
E. Prats;M. Noël;J. Letourneau;V. Tiranti;J. Vaqué;R. Debón;M. Zeviani;L. Cornudella;A. Ruiz
内切酶G （Endo G）是一种在脊椎动物线粒体中发现的原核谱系的核酸酶，已被认为在线粒体DNA （mtDNA）复制中发挥作用。我们分离并测序了整个小鼠endo G基因，确定了mRNA的极限，并绘制了启动子区域。单拷贝基因的编码序列被两个内含子打断，转录本分析不支持一个以上的Endo G异构体可以通过选择性剪接产生的模型。我们还鉴定了一个全长的人类Endo G cDNA，并在蛋白质水平上比较了人类、牛和小鼠的核酸酶，表明除了各自的线粒体靶向信号外，它们具有高度的保守性。Endo G普遍表达，其mRNA的稳态水平在不同组织之间的差异大于7个因子。mtDNA拷贝数与Endo G mRNA水平之间的关系并不严格成正比，但mtDNA含量丰富的组织mRNA含量较高，反之亦然。
Endonuclease G (Endo G) is a nuclease of prokaryotic lineage found in the mitochondria of vertebrates that has been suggested to play a role in mitochondrial DNA (mtDNA) replication. We have isolated and sequenced the entire mouse endo G gene, determined the limits of the mRNA, and mapped the promoter region. The coding sequence of the single copy gene is interrupted by two introns and analysis of the transcripts does not support a model by which more than one Endo G isoform could be produced by alternative splicing. We have also characterized a full-length human Endo G cDNA and comparison at the protein level of the human, bovine, and murine nucleases indicates a high degree of conservation except in the respective mitochondrial targeting signals. Endo G is ubiquitously expressed and the steady-state levels of its mRNA vary by a factor greater than seven between different tissues. The relationship between the mtDNA copy number and Endo G mRNA levels is not strictly proportional but tissues richer in mtDNA have higher amounts of the mRNA and vice versa.
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DOI：
10.1016/s0021-9258(18)67252-7
发表时间：
1986-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Williams
通讯作者：
R. Williams
NUC1（酿酒酵母中编码线粒体核酸酶的基因）的序列和表达。
影响因子：
14.9
作者：
Vincent,RD;Hofmann,TJ;Zassenhaus,HP
通讯作者：
Zassenhaus,HP
细胞质和线粒体 tRNA 核苷酸转移酶活性源自酿酒酵母中的同一基因。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen,JY;Joyce,PB;Wolfe,CL;Steffen,MC;Martin,NC
通讯作者：
Martin,NC
DOI：
10.1073/pnas.89.13.5814
发表时间：
1992-07
影响因子：
11.1
作者：
Steven R. Wiley;Richard J. Kraus;Janet E. Mertz
通讯作者：
Steven R. Wiley;Richard J. Kraus;Janet E. Mertz
影响因子：
10.5
作者：
VIRBASIUS, CMA;VIRBASIUS, JV;SCARPULLA, RC
通讯作者：
SCARPULLA, RC