Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes.
Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes.
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DOI：
10.1038/s41598-018-32758-8
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发表时间：
2018-09-27
影响因子：
4.6
通讯作者：
Kataura H
中科院分区：
文献类型：
--
作者：
Yudasaka M;Yomogida Y;Zhang M;Nakahara M;Kobayashi N;Tanaka T;Okamatsu-Ogura Y;Saeki K;Kataura H
棕色脂肪组织（BAT）是由产热棕色脂肪细胞（BA）和非实质成分（包括血管和细胞外基质）组成，有助于维持体温。BAT分布通过使用18 F-氟脱氧葡萄糖（18 F-FDG）的正电子发射断层扫描-计算机断层扫描（PET/CT）或使用[123/125 I]-β-甲基-对碘苯基-十五烷酸的单光子发射计算机断层扫描-计算机断层扫描（SPECT/CT）检测。虽然交感神经活动和产热能力的BA在小鼠禁食条件下下调，禁食依赖的结构变化和流体动力学的BAT仍然未知。在这里，我们表明，禁食诱导精细和可逆的结构变化，在非实质区域在小鼠BAT与加宽的细胞间隙和变形的胶原带，如电子显微镜所示。有趣的是，一种新引入的近红外荧光探针的单壁碳纳米管（CNT）涂有磷脂聚乙二醇（PLPEG）很容易证明增强血管通透性的BAT的禁食。PLPEG-CNT外渗并保留在细胞间隙中或进一步重新分布在禁食小鼠的实质细胞中，这是以前未知的现象。因此，PLPEG-CNT提供了一个强大的工具来跟踪亚组织水平的流体动力学。
Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/CT) using 18F-fluorodeoxy glucose (18F-FDG) or single-photon-emission computed tomography-computed tomography (SPECT/CT) using [123/125I]-beta-methyl-p-iodophenyl-pentadecanoic acid. Although sympathetic nerve activity and thermogenic capacity of BA is downregulated under fasting conditions in mice, fasting-dependent structural changes and fluid kinetics of BAT remain unknown. Here we show that the fasting induces fine and reversible structural changes in the non-parenchymal region in murine BAT with widened intercellular spaces and deformed collagen bands as revealed by electron microscopy. Interestingly, a newly introduced near infrared fluorescent probe of single-walled carbon nanotubes (CNTs) coated with phospholipid polyethylene glycol (PLPEG) easily demonstrated enhanced vascular permeability in BAT by the fasting. PLPEG-CNTs extravasated and remained in intercellular spaces or further redistributed in parenchymal cells in fasted mice, which is a previously unknown phenomenon. Thus, PLPEG-CNTs provide a powerful tool to trace fluid kinetics in sub-tissue levels.
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DOI：
10.1186/1745-7580-4-5
发表时间：
2008-04-29
期刊：
Immunome research
影响因子：
--
作者：
Lattin JE;Schroder K;Su AI;Walker JR;Zhang J;Wiltshire T;Saijo K;Glass CK;Hume DA;Kellie S;Sweet MJ
通讯作者：
Sweet MJ
影响因子：
7.7
作者：
Saito M;Okamatsu-Ogura Y;Matsushita M;Watanabe K;Yoneshiro T;Nio-Kobayashi J;Iwanaga T;Miyagawa M;Kameya T;Nakada K;Kawai Y;Tsujisaki M
通讯作者：
Tsujisaki M
影响因子：
56.9
作者：
O'Connell, MJ;Bachilo, SM;Smalley, RE
通讯作者：
Smalley, RE
影响因子：
4
作者：
Lichtenstein, Alexandra;Minogue, Peter J.;Berthoud, Viviana M.
通讯作者：
Berthoud, Viviana M.
影响因子：
64.8
作者：
IIJIMA, S
通讯作者：
IIJIMA, S