Down-regulated expression of miR-99a is associated with lymph node metastasis and predicts poor outcome in stage IB cervical squamous cell carcinoma: a case-control study.
Down-regulated expression of miR-99a is associated with lymph node metastasis and predicts poor outcome in stage IB cervical squamous cell carcinoma: a case-control study.
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DOI：
10.21037/atm-22-2483
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发表时间：
2022-06
影响因子：
--
通讯作者：
Wu, Lingying
中科院分区：
文献类型：
--
作者：
Zhang, Gongyi;Zhang, Rong;Bai, Ping;Li, Shumin;Zuo, Jing;Zhang, Yuanyuan;Liu, Mei;Wu, Lingying
淋巴结转移是宫颈癌最重要的转移途径。然而，在具有相似临床病理变量的患者中，LNM的状态不同。研究发现，microrna广泛参与各种恶性肿瘤的发生和发展，microRNA-99 （miR-99）家族的肿瘤抑制或促进作用也有报道。本研究旨在探讨miR-99a对淋巴性扩散的预测价值及其对早期宫颈鳞状细胞癌（CSCC）患者生存的影响。研究纳入了2015年10月至2018年11月期间接受手术治疗的IB期鳞状宫颈癌患者。取病理证实的阳性淋巴结经福尔马林固定石蜡包埋组织21例，另取基线特征匹配的阴性淋巴结组织21例作为对照组。采用TaqMan实时定量聚合酶链反应检测样品中miR-99a的表达水平。采用独立样本t检验比较两组间miR-99a的差异表达水平。此外，采用卡方检验或Fisher精确概率法评估miR-99a表达水平与42例患者临床病理参数的相关性，并采用Kaplan-Meier积限法评估其对生存的影响。两组患者基线临床病理参数比较，差异均无统计学意义（P < 0.05）。miR-99a在淋巴结阳性组和对照组的表达水平分别为1.61±3.09和16.77±30.40，差异有统计学意义（P=0.029）。miR-99a表达下调与浸润深度（DOI）和淋巴血管间隙浸润密切相关（P<0.05）。单因素分析显示miR-99a表达下调、DOI越深与5年无病生存期越差相关，多因素分析显示miR-99a表达水平是影响无病生存期的独立因素（HR =0.120; 95% CI: 0.015 ~ 0.979； P=0.048）。miR-99a下调的患者总体生存期更不利，但差异无统计学意义。MiR-99a在淋巴结转移的发病机制中发挥抑制作用，可能作为CSCC患者新的预后生物标志物。
Lymph node metastasis (LNM) accounts for the most important route of metastasis for cervical cancer. Yet, the status of LNM is different in patients with similar clinico-pathological variables. It has been revealed that microRNAs are widely involved in the occurrence and development of various malignancies, and the tumor-suppressive or promoting effects of microRNA-99 (miR-99) family have been previously reported. This study sought to investigate the predictive value of miR-99a for lymphogenous spread and its effect on the survival of patients with early-stage cervical squamous cell cancer (CSCC). Patients with stage IB squamous cervical cancer who were treated surgically between October 2015 and November 2018 were enrolled. A total of 21 formalin-fixed paraffin-embedded tissues of pathologically confirmed positive lymph nodes were retrieved, and an additional 21 tissues of negative lymph nodes from patients well-matched on baseline characteristics were collected as the control group. TaqMan real-time quantitative polymerase chain reaction was used to examine the expression levels of miR-99a in the samples. Differential expression levels of miR-99a were compared between the 2 groups using independent sample t-test. Furthermore, the associations between miR-99a expression level and clinico-pathological parameters of these 42 patients was evaluated by Chi-square test or Fisher’s exact-probability method, and their effects on survival were assessed using Kaplan-Meier product-limit method. There were no significant differences in baseline clinico-pathological parameters between the 2 groups (P>0.05). The expression levels of miR-99a in the node-positive group and control group were 1.61±3.09 and 16.77±30.40, respectively (P=0.029). Downregulated expression of miR-99a was closely related to depth of invasion (DOI) and lymph-vascular space invasion (P<0.05). Univariate analysis revealed that downregulated miR-99a and deeper DOI were associated with worse 5-year disease-free survival, while multivariate analysis showed that only the expression level of miR-99a was an independent factor for disease-free survival (HR =0.120; 95% CI: 0.015–0.979; P=0.048). Patients with downregulated miR-99a tended to have more unfavorable overall survival, but the difference did not reach statistical significance. MiR-99a plays an inhibitory role in the pathogenesis of lymph node metastasis and may serve as a novel prognostic biomarker for patients with CSCC.
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影响因子：
2.9
作者：
Cui J;Pan Y;Wang J;Liu Y;Wang H;Li H
通讯作者：
Li H
DOI：
10.1056/nejmoa1309748
发表时间：
2014-02-20
期刊：
The New England journal of medicine
影响因子：
--
作者：
Tewari KS;Sill MW;Long HJ 3rd;Penson RT;Huang H;Ramondetta LM;Landrum LM;Oaknin A;Reid TJ;Leitao MM;Michael HE;Monk BJ
通讯作者：
Monk BJ
影响因子：
2.4
作者：
Nanthamongkolkul, Kulisara;Hanprasertpong, Jitti
通讯作者：
Hanprasertpong, Jitti
影响因子：
3.4
作者：
Wang, Li;Chang, Lihua;Li, Mu
通讯作者：
Li, Mu
影响因子：
4
作者：
Zhao J;Chen F;Zhou Q;Pan W;Wang X;Xu J;Ni L;Yang H
通讯作者：
Yang H