Juvenile Spermatogonial Depletion (jsd) Mutant Seminiferous Tubules Are Capable of Supporting Transplanted Spermatogenesis1
Juvenile Spermatogonial Depletion (jsd) Mutant Seminiferous Tubules Are Capable of Supporting Transplanted Spermatogenesis1
复制标题
幼年精原细胞耗竭 (jsd) 突变的曲细精管能够支持移植的精子发生1
作者：
H. Boettger;D. Johnston;L. Russell;M. Griswold;C. Bishop
摘要在小鼠中，幼年精原耗竭(JSD)突变导致单波精子发生，随后A型精原干细胞无法重新填充睾丸，导致雄性动物不育。目前尚不清楚JSD的缺陷是由于躯体成分未能支持精子发生，还是存在于突变的生殖细胞固有的失败。为了确定JSD睾丸内环境是否能够支持精子发生，进行了生殖细胞移植实验，其中C57BL/6玫瑰生殖细胞被移植到JSD受体。为了确定JSD精原细胞是否能够在允许的生精环境中发育，将JSD生殖细胞移植到W/WV和经白消丹处理的C57BL/6动物体内。这些数据表明，在正常生殖细胞移植后7个月，JSD生精小管能够支持生精。相反，将JSD生殖细胞移植到经白消丹处理的C57BL/6小鼠或W/WV小鼠的睾丸中，没有观察到JSD来源的精子发生。这些数据支持这样的假设，即JSD的表型是由于生殖细胞本身的缺陷，而不是肾小管内环境的缺陷。
Abstract In mice, the juvenile spermatogonial depletion (jsd) mutation results in a single wave of spermatogenesis followed by failure of type A spermatogonial stem cells to repopulate the testis, rendering male animals sterile. It is not clear whether the defect in jsd resides in a failure of the somatic component to support spermatogenesis or in a failure that is intrinsic to the mutant's germ cells. To determine if the jsd intratesticular environment is capable of supporting spermatogenesis, germ cell transplantation experiments were performed in which C57BL/6 ROSA germ cells were transplanted into jsd recipients. To determine if jsd spermatogonia are able to develop in a permissive seminiferous environment, jsd germ cells were transplanted into W/Wv and busulfan-treated C57BL/6 animals. The data demonstrate that up to 7 mo after transplantation of normal germ cells, jsd seminiferous tubules are capable of supporting spermatogenesis. In contrast, when jsd germ cells were transplanted into busulfan-treated C57BL/6 testis, or into testis of W/Wv mice, no jsd-derived spermatogenesis was observed. The data support the hypothesis that the jsd phenotype is due to a defect in the germ cells themselves, and not in the intratubular environment.
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影响因子：
10.5
作者：
Guang-Quan Zhao;Ke-Yu Deng;P. Labosky;Lucy Liaw;Brigid L. M. Hogan
通讯作者：
Guang-Quan Zhao;Ke-Yu Deng;P. Labosky;Lucy Liaw;Brigid L. M. Hogan
影响因子：
--
作者：
Lonnie D. Russell;L. R. França;R. Brinster
通讯作者：
Lonnie D. Russell;L. R. França;R. Brinster
DOI：
10.1387/ijdb.9074943
发表时间：
2003-02
期刊：
The International journal of developmental biology
影响因子：
--
作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
通讯作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
DOI：
10.1073/pnas.91.24.11303
发表时间：
1994-11-22
影响因子：
11.1
作者：
BRINSTER, RL;AVARBOCK, MR
通讯作者：
AVARBOCK, MR
通过促性腺激素释放激素拮抗剂治疗刺激幼年精原细胞耗竭（jsd）突变小鼠的精原细胞分化。
影响因子：
4.8
作者：
Matsumiya,K;Meistrich,ML;Shetty,G;Dohmae,K;Tohda,A;Okuyama,A;Nishimune,Y
通讯作者：
Nishimune,Y