Complex sputum microbial composition in patients with pulmonary tuberculosis.
Complex sputum microbial composition in patients with pulmonary tuberculosis.
复制标题
DOI：
10.1186/1471-2180-12-276
复制
发表时间：
2012-11-23
期刊：
影响因子：
4.2
通讯作者：
Guo X
中科院分区：
文献类型：
--
作者：
Cui Z;Zhou Y;Li H;Zhang Y;Zhang S;Tang S;Guo X
越来越多的研究表明，微生物组与某些疾病，特别是慢性疾病有关。本研究对肺结核患者痰中的细菌群落进行了研究。从31例肺结核患者的痰液和24例健康人的呼吸道分泌物中提取总DNA。使用条形码引物扩增16S rRNA V3高变区，并使用Roche 454 FLX进行焦磷酸测序。结果表明，肺结核患者痰菌群的多样性高于健康人（P<0.05）。这些序列被分为24个门，所有这些都在肺结核患者中发现，其中17个在健康参与者中发现。此外，许多外来细菌，如寡养单胞菌属、贪铜菌属、假单胞菌属、栖热菌属、鞘氨醇单胞菌属、甲基杆菌属、黄单胞菌属、丛毛单胞菌属和移动球菌属，是肺结核患者所特有的。本研究得出结论，肺结核患者呼吸道的微生物组成比健康参与者复杂，在肺结核患者的痰中发现了许多外来细菌。这些外来细菌在肺结核发病或发展中的作用应引起临床医师的重视。
An increasing number of studies have implicated the microbiome in certain diseases, especially chronic diseases. In this study, the bacterial communities in the sputum of pulmonary tuberculosis patients were explored. Total DNA was extracted from sputum samples from 31 pulmonary tuberculosis patients and respiratory secretions of 24 healthy participants. The 16S rRNA V3 hyper-variable regions were amplified using bar-coded primers and pyro-sequenced using Roche 454 FLX. The results showed that the microbiota in the sputum of pulmonary tuberculosis patients were more diverse than those of healthy participants (p<0.05). The sequences were classified into 24 phyla, all of which were found in pulmonary tuberculosis patients and 17 of which were found in healthy participants. Furthermore, many foreign bacteria, such as Stenotrophomonas, Cupriavidus, Pseudomonas, Thermus, Sphingomonas, Methylobacterium, Diaphorobacter, Comamonas, and Mobilicoccus, were unique to pulmonary tuberculosis patients. This study concluded that the microbial composition of the respiratory tract of pulmonary tuberculosis patients is more complicated than that of healthy participants, and many foreign bacteria were found in the sputum of pulmonary tuberculosis patients. The roles of these foreign bacteria in the onset or development of pulmonary tuberculosis shoud be considered by clinicians.
登录
查看更多内容
DOI：
10.2174/1874306401004010076
发表时间：
2010-08-08
期刊：
The open respiratory medicine journal
影响因子：
--
作者：
Khan, Zinobia;Miller, Albert;Donath, Joseph
通讯作者：
Donath, Joseph
DOI：
10.1073/pnas.1019378108
发表时间：
2011-03-29
影响因子：
11.1
作者：
Ichinohe, Takeshi;Pang, Iris K.;Iwasaki, Akiko
通讯作者：
Iwasaki, Akiko
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
3.6
作者：
Ling, Zongxin;Liu, Xia;Xiang, Charlie
通讯作者：
Xiang, Charlie
下呼吸道感染患者痰标本微生物群分析
影响因子：
3.7
作者：
Zhou, Yuhua;Lin, Ping;Guo, Xiaokui
通讯作者：
Guo, Xiaokui