Genetic control of water use efficiency and leaf carbon isotope discrimination in sunflower (Helianthus annuus L.) subjected to two drought scenarios.
Genetic control of water use efficiency and leaf carbon isotope discrimination in sunflower (Helianthus annuus L.) subjected to two drought scenarios.
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葵花籽（Helianthus annuus L.）中用水效率和叶碳同位素歧视的遗传控制。
DOI：
10.1371/journal.pone.0101218
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Grieu P
中科院分区：
文献类型：
--
作者：
Adiredjo AL;Navaud O;Muños S;Langlade NB;Lamaze T;Grieu P
通过协调生物量积累和水分消耗，可以获得较高的水分利用效率。植物叶片中的WUE与碳同位素识别（CID）在生理和遗传上相关。以148个由XRQ和PSC8杂交获得的向日葵重组自交系（RIL）群体为研究对象，分析了不同干旱条件下控制向日葵水分利用效率和CID的数量性状基因座（QTL），并比较了这些性状的QTL定位。我们在2011年和2012年进行了温室实验，使用100个天平，每天测量水分蒸腾，我们确定了WUE，CID，生物量和植物的累积水分蒸腾。在两个试验中观察到广泛的表型变异，显着的基因型效应，WUE和CID之间的显着负相关。在两个试验中共鉴定出9个控制WUE的QTL和8个控制CID的QTL。还显著鉴定了控制WUE和CID的表型反应QTL。WUE的QTL是干旱情景所特有的，而CID的QTL是独立的干旱情景，可以在所有的实验。我们的结果表明，控制CID的稳定基因组区域位于连锁群06和13（LG 06和LG 13）上。3个CID QTL与WUE、生物量和累积耗水量QTL共定位。我们发现CID和WUE高度相关，有共同的遗传控制。有趣的是，这些性状的遗传控制表现出与环境的相互作用（在两种干旱情景和控制条件之间）。研究结果为利用CID和分子标记辅助育种技术选育高水分利用效率品种开辟了一条新途径，有助于保持向日葵作物生产的稳定性。
High water use efficiency (WUE) can be achieved by coordination of biomass accumulation and water consumption. WUE is physiologically and genetically linked to carbon isotope discrimination (CID) in leaves of plants. A population of 148 recombinant inbred lines (RILs) of sunflower derived from a cross between XRQ and PSC8 lines was studied to identify quantitative trait loci (QTL) controlling WUE and CID, and to compare QTL associated with these traits in different drought scenarios. We conducted greenhouse experiments in 2011 and 2012 by using 100 balances which provided a daily measurement of water transpired, and we determined WUE, CID, biomass and cumulative water transpired by plants. Wide phenotypic variability, significant genotypic effects, and significant negative correlations between WUE and CID were observed in both experiments. A total of nine QTL controlling WUE and eight controlling CID were identified across the two experiments. A QTL for phenotypic response controlling WUE and CID was also significantly identified. The QTL for WUE were specific to the drought scenarios, whereas the QTL for CID were independent of the drought scenarios and could be found in all the experiments. Our results showed that the stable genomic regions controlling CID were located on the linkage groups 06 and 13 (LG06 and LG13). Three QTL for CID were co-localized with the QTL for WUE, biomass and cumulative water transpired. We found that CID and WUE are highly correlated and have common genetic control. Interestingly, the genetic control of these traits showed an interaction with the environment (between the two drought scenarios and control conditions). Our results open a way for breeding higher WUE by using CID and marker-assisted approaches and therefore help to maintain the stability of sunflower crop production.
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影响因子：
5.4
作者：
Al-Chaarani, GR;Gentzbittel, L;Sarrafi, A
通讯作者：
Sarrafi, A
影响因子：
5.4
作者：
Diab, AA;Teulat-Merah, B;Sorrells, ME
通讯作者：
Sorrells, ME
影响因子：
4.2
作者：
Blum, A
通讯作者：
Blum, A
影响因子：
7.3
作者：
Chen, Jing;Chang, Scott X.;Anyia, Anthony O.
通讯作者：
Anyia, Anthony O.
影响因子：
--
作者：
CONDON, AG;RICHARDS, RA;FARQUHAR, GD
通讯作者：
FARQUHAR, GD