The ability of Clostridium bifermentans strains to lactic acid biosynthesis in various environmental conditions.
The ability of Clostridium bifermentans strains to lactic acid biosynthesis in various environmental conditions.
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DOI：
10.1186/2193-1801-2-44
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Czaczyk K
中科院分区：
文献类型：
--
作者：
Leja K;Myszka K;Czaczyk K
对从粪便中分离的双发酵梭菌在不同pH条件下和添加果糖、山梨醇、葡萄糖、甘露糖、甘露醇、麦芽糖、木糖、棉子糖和阿拉伯糖的PY培养基产乳酸能力进行了研究。在最后一次测试中，检查了被调查的菌株从混合碳源(甘油+糖)中产生乳酸的能力。氯化细菌的菌株。分别命名为CB 371、CB 374和CB 376的双发酵菌在PY培养基上生长并产生乳酸，而不受pH值和所用碳源的影响。在含有甘油的PY培养基中，Cb371和Cb376的最适pH分别为7.0(19.63g/L和16.65g/L)，Cb374的最适pH为8.0(13.88g/L)。以甘露醇为碳源时，CB371、CB374和CB376菌株在PY培养基产乳酸效果最好(30g/L以上)。混合碳源对氯化产酸没有明显的促进作用。双酵母菌。乳酸的产率与在单独使用甘油的条件下的乳酸产量大致相同，但低于在单独使用甘露醇的条件下的乳酸产量。因此，从环境的角度来看，只使用甘油等废旧材料的介质更有利。本文的在线版本(DOI：10.1186/2193-1801-2-44)包含补充材料，可供授权用户使用。
Clostridium bifermentans strains, isolated from a manure, were examinated for their ability to produce lactic acid from PY medium with glycerol under different pH conditions and when PY medium was supplemented with saccharides such as fructose, sorbitol, glucose, mannose, mannitol, maltose, xylose, raffinose, and arabinose. In the last test performed, the ability of investigated strains to produce lactic acid from mixed carbon source (glycerol plus saccharide) was checked. The strains of Cl. bifermentans, designated as CB 371, CB 374, and CB 376 grew and produced lactic acid on PY medium irrespective of pH and the carbon source used. The optimal lactic acid production on PY medium with glycerol was obtained at pH of 7.0 in case of CB 371 and 376 (19.63 g/L and 16.65 g/L, accordingly) and at pH 8.0 in case of CB 374 (13.88 g/L). The best productivity of lactic acid on PY media by CB 371, CB 374, and CB 376 (above 30 g/L) was observed when mannitol was used as a carbon source. The mixed carbon source did not increase productivity of lactic acid by Cl. bifermentans. The yield of lactic acid was approximately equal to the yield of lactic acid obtained on the medium with only glycerol and lower than in medium with only mannitol. Thus, from the environmental point of view it is more beneficial to use the medium with waste-type material only, such as glycerol. The online version of this article (doi:10.1186/2193-1801-2-44) contains supplementary material, which is available to authorized users.
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DOI：
10.1002/ep.10225
发表时间：
2007-12-01
期刊：
ENVIRONMENTAL PROGRESS
影响因子：
--
作者：
Johnson, Duane T.;Taconi, Katherine A.
通讯作者：
Taconi, Katherine A.
影响因子：
11.4
作者：
Ruhal, Rohit;Choudhury, Bijan
通讯作者：
Choudhury, Bijan
影响因子：
2.9
作者：
Rahman, KSM;Rahman, TJ;Banat, IM
通讯作者：
Banat, IM
影响因子：
3.2
作者：
Clark, FE
通讯作者：
Clark, FE
影响因子：
5
作者：
Ringel, Anne Katrin;Wilkens, Erik;Vorlop, Klaus-Dieter
通讯作者：
Vorlop, Klaus-Dieter