Oestrogen receptor negative breast cancers exhibit high cytokine content.
Oestrogen receptor negative breast cancers exhibit high cytokine content.
复制标题
DOI：
10.1186/bcr1648
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Lazennec G
中科院分区：
文献类型：
--
作者：
Chavey C;Bibeau F;Gourgou-Bourgade S;Burlinchon S;Boissière F;Laune D;Roques S;Lazennec G
一个新的假设表明，细胞因子作为血管生成和白细胞浸润的潜在调节剂，可能在癌症中发挥重要作用。采用新型多重流式细胞术技术测定 17 种细胞因子（IL-1β、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-10、IL-12 [p70]、IL-13、IL-17、粒细胞集落刺激因子 [CSF]、粒细胞巨噬细胞 CSF、IFN-γ、单核细胞趋化蛋白）的表达105 例乳腺癌中蛋白质水平的[MCP]-1、巨噬细胞炎症蛋白[MIP]-1β、肿瘤坏死因子[TNF]-α）。通过免疫组织化学测定B淋巴细胞、T淋巴细胞和巨噬细胞水平。 17 种细胞因子中有 14 种在乳腺癌中表达，而在正常乳腺中仅检测到 9 种细胞因子。大多数细胞因子在乳腺癌中比正常乳腺中更丰富，其中IL-6、IL-8、粒细胞CSF、IFN-γ、MCP-1和MIP-1β非常丰富。 IL-2、IL-6、IL-8、IL-10、IFN-γ、MCP-1、MIP-1β 和 TNF-α，以及较小程度的 IL-1β 和 IL-13 表现出与雌激素受体和孕激素受体状态呈负相关的表达水平。大多数细胞因子与癌症诊断时的年龄、肿瘤大小、组织学类型或淋巴结状态无关。然而，IL-1β、IL-6、IL-8、IL-10、IL-12、MCP-1 和 MIP-1β 在高级别肿瘤中比在低级别肿瘤中更丰富。此外，IL-8和MIP-1β在HER2阳性患者中的表达程度高于HER2阴性患者。大多数研究的细胞因子的表达与激活蛋白 1 的水平相关，已知激活蛋白 1 可以调节多种细胞因子。 MCP-1和MIP-1β的过表达与B淋巴细胞、T淋巴细胞和巨噬细胞浸润有关，而高水平的IL-8与肿瘤中高巨噬细胞含量相关。此外，IL-8阳性肿瘤表现出血管化增加。我们发现多种细胞因子在雌激素受体阴性乳腺癌中过度表达，并且三种主要细胞因子——MCP-1、MIP-1β和IL-8——与炎症细胞成分相关，这可以解释这些肿瘤的侵袭性。
An emerging hypothesis suggests that cytokines could play an important role in cancer as potential modulators of angiogenesis and leucocyte infiltration. A novel multiplexed flow cytometry technology was used to measure the expression of 17 cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 [p70], IL-13, IL-17, granulocyte colony-stimulating factor [CSF], granulocyte-macrophage CSF, IFN-γ, monocyte chemoattractant protein [MCP]-1, macrophage inflammatory protein [MIP]-1β, tumour necrosis factor [TNF]-α) at the protein level in 105 breast carcinoma. B lymphocyte, T lymphocyte and macrophage levels were determined by immunohistochemistry. Fourteen of the 17 cytokines were expressed in breast carcinoma, whereas only nine cytokines could be detected in normal breast. Most cytokines were more abundant in breast carcinoma than in normal breast, with IL-6, IL-8, granulocyte CSF, IFN-γ, MCP-1 and MIP-1β being very abundant. IL-2, IL-6, IL-8, IL-10, IFN-γ, MCP-1, MIP-1β and TNF-α, and to a lesser extent IL-1β and IL-13 exhibited levels of expression that were inversely correlated to oestrogen receptor and progesterone receptor status. Most cytokines were not correlated with age at cancer diagnosis, tumour size, histological type, or lymph node status. However, IL-1β, IL-6, IL-8, IL-10, IL-12, MCP-1 and MIP-1β were more abundant in high-grade tumours than in low-grade tumours. In addition, IL-8 and MIP-1β were expressed to a greater degree in HER2-positive than in HER2-negative patients. The expression of most of the studied cytokines was correlated to levels of activator protein-1, which is known to regulate numerous cytokines. Overexpression of MCP-1 and MIP-1β were linked to B lymphocyte, T lymphocyte and macrophage infiltration, whereas high levels of IL-8 were correlated with high macrophage content in tumour. Moreover, IL-8 positive tumours exhibited increased vascularization. We found that multiple cytokines were overexpressed in oestrogen receptor negative breast carcinoma, and that the three major cytokines – MCP-1, MIP-1β and IL-8 – were correlated with inflammatory cell component, which could account for the aggressiveness of these tumours.
登录
查看更多内容
DOI：
10.1006/bbrc.1997.7502
发表时间：
1997-10-20
影响因子：
3.1
作者：
Kelly, RW;Carr, GG;Riley, SC
通讯作者：
Riley, SC
影响因子：
4
作者：
Carruba, G;D'Agostino, P;Cillari, E
通讯作者：
Cillari, E
影响因子：
2.4
作者：
Miller, LJ;Kurtzman, SH;Kreutzer, DL
通讯作者：
Kreutzer, DL
影响因子：
254.7
作者：
Parkin, DM;Bray, F;Pisani, P
通讯作者：
Pisani, P
影响因子：
3.8
作者：
McMurray, RW;Ndebele, K;Jenkins, JK
通讯作者：
Jenkins, JK