Tubeimoside-1 attenuates LPS-induced inflammation in RAW 264.7 macrophages and mouse models
Tubeimoside-1 attenuates LPS-induced inflammation in RAW 264.7 macrophages and mouse models
复制标题
Tubeimoside-1 可减轻 RAW 264.7 巨噬细胞和小鼠模型中 LPS 诱导的炎症
DOI：
10.3109/08923973.2013.810643
复制
发表时间：
2013-07
影响因子：
3.3
通讯作者：
Feng, Haihua
中科院分区：
文献类型：
--
作者：
Soromou, Lanan Wassy;Chen, Na;Xiong, Ying;Feng, Haihua
摘要背景：急性肺损伤（Acute lung injury， ALI）是一种常见的临床疾病，以严重低氧血症、肺水肿和肺中性粒细胞积聚为特征，在休克、败血症、缺血再灌注等方面具有较高的发病率和死亡率。目的：在本研究中，我们旨在研究tubeimoside-1 （TBMS1）对脂多糖（LPS）刺激的RAW 264.7细胞炎症的保护作用和脂多糖诱导的肺损伤模型。材料和方法：采用酶联免疫吸附法评价TBMS1对lps诱导的RAW 264.7细胞培养上清中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6和IL-1β产生的影响。将LPS （0.5 mg/kg）滴入磷酸盐缓冲盐水中诱导ALI，并在LPS刺激后6 h评估肺损伤的严重程度。结果：TBMS1在体外和体内均能显著抑制促炎细胞因子TNF-α、IL-6和IL-1β的产生。TBMS1预处理可显著减轻ALI小鼠肺水肿的发展、组织学严重程度和炎症细胞浸润。此外，我们进一步证明TBMS1通过抑制i- κ b活化和p38/细胞外信号调节的激酶丝裂原活化蛋白激酶信号通路，以剂量依赖的方式在ALI体内模型中发挥抗炎作用。讨论和结论：总的来说，我们的数据表明TBMS1在体外和体内都能抑制炎症，可能是预防炎症性疾病的潜在治疗候选药物。
Abstract Context: Acute lung injury (ALI), characterized by severe hypoxemia, pulmonary edema and neutrophil accumulation in the lung, is a common clinical problem associated with significant morbidity and mortality in shock, sepsis, ischemia reperfusion, etc. Objective: In this study, we aimed at investigating the protective effect of tubeimoside-1 (TBMS1) on inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a LPS-induced in vivo lung injury model. Materials and methods: We evaluated the effect of TBMS1 on LPS-induced production of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β in the culture supernatants of RAW 264.7 cells by enzyme-linked immunosorbent assay. LPS (0.5 mg/kg) was instilled intranasally in phosphate-buffered saline to induce ALI, and the severity of pulmonary injury was evaluated 6 h after LPS challenge. Results: TBMS1 significantly inhibited the production of the pro-inflammatory cytokines, TNF-α, IL-6 and IL-1β in vitro and in vivo. Pretreatment with TBMS1 markedly attenuated the development of pulmonary edema, histological severities and inflammatory cells infiltration in mice with ALI. In addition, we further demonstrated that TBMS1 exerts an anti-inflammatory effect in vivo model of ALI through suppression of IκB activation and p38/extracellular signal-regulated kinase mitogen-activated protein kinases signaling in a dose-dependent manner. Discussion and conclusion: Overall, our data suggest that TBMS1 inhibits inflammation both in vitro and in vivo, and may be a potential therapeutic candidate for the prevention of inflammatory diseases.
登录
查看更多内容
影响因子：
3.7
作者：
M. Bocchino;A. Marruchella;C. Saltini
通讯作者：
M. Bocchino;A. Marruchella;C. Saltini
影响因子：
3.8
作者：
通讯作者：
--
影响因子：
5.8
作者：
Schuh, Katrin;Pahl, Andreas
通讯作者：
Pahl, Andreas
影响因子：
4.2
作者：
Berenbaum, F
通讯作者：
Berenbaum, F
影响因子：
5.8
作者：
Rahman, I
通讯作者：
Rahman, I