Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast.
Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast.
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DOI：
10.1091/mbc.e09-05-0412
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发表时间：
2010-04-01
影响因子：
3.3
通讯作者：
Tanaka K
中科院分区：
文献类型：
--
作者：
Yamamoto T;Mochida J;Kadota J;Takeda M;Bi E;Tanaka K
芽殖酵母在肌动蛋白电缆组装中的突变体仍然可以形成小的芽，肌动蛋白电缆被认为是芽殖所必需的唯一肌动蛋白结构。肌动蛋白补丁内吞机制/内吞再循环因子的突变抑制了这种出芽，这表明了一种机制，即通过内吞囊泡的局部再循环促进极化生长。丝状肌动蛋白的组装对于芽殖酵母中极化芽的生长是必不可少的。肌动蛋白电缆，这是组装的formins Bni 1 p和Bnr 1 p，被认为是唯一的肌动蛋白结构，是必不可少的萌芽。然而，我们发现缺乏肌动蛋白索的原肌球蛋白突变体仍然能够形成一个小的芽。皮层肌动蛋白补丁，这是由Arp 2/3复合物组装的内吞囊泡形成中发挥关键作用的组件中的额外突变，抑制这种萌芽。参与内吞再循环的基因也需要在肌动蛋白电缆少突变体的小芽形成。这些结果表明，芽殖酵母具有一种机制，通过局部回收内吞囊泡促进极化生长。有趣的是，V型肌球蛋白Myo 2 p，被认为只使用肌动蛋白电缆跟踪，也有助于在缺乏肌动蛋白电缆的情况下出芽。这些结果表明，一些肌动蛋白网络可能作为轨道Myo 2 p驱动的囊泡运输在肌动蛋白电缆的情况下，或Myo 2 p可以独立于肌动蛋白丝的功能。我们的研究结果还表明，极性监管机构，包括Cdc 42 p仍然极化缺陷的肌动蛋白电缆和皮层肌动蛋白补丁的突变体，这表明肌动蛋白细胞骨架不发挥主要作用，在皮层组装的极性监管机构在芽殖酵母。
Budding yeast mutants in assembly of actin cables, which are thought to be the only actin structures essential for budding, still could form a small bud. Mutations in actin patch endocytic machineries/endocytic recycling factors inhibited this budding, suggesting a mechanism that promotes polarized growth by local recycling of endocytic vesicles. The assembly of filamentous actin is essential for polarized bud growth in budding yeast. Actin cables, which are assembled by the formins Bni1p and Bnr1p, are thought to be the only actin structures that are essential for budding. However, we found that formin or tropomyosin mutants, which lack actin cables, are still able to form a small bud. Additional mutations in components for cortical actin patches, which are assembled by the Arp2/3 complex to play a pivotal role in endocytic vesicle formation, inhibited this budding. Genes involved in endocytic recycling were also required for small-bud formation in actin cable-less mutants. These results suggest that budding yeast possesses a mechanism that promotes polarized growth by local recycling of endocytic vesicles. Interestingly, the type V myosin Myo2p, which was thought to use only actin cables to track, also contributed to budding in the absence of actin cables. These results suggest that some actin network may serve as the track for Myo2p-driven vesicle transport in the absence of actin cables or that Myo2p can function independent of actin filaments. Our results also show that polarity regulators including Cdc42p were still polarized in mutants defective in both actin cables and cortical actin patches, suggesting that the actin cytoskeleton does not play a major role in cortical assembly of polarity regulators in budding yeast.
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酵母A因子受体的回收。
影响因子：
7.8
作者：
Chen, L;Davis, N G
通讯作者：
Davis, N G
影响因子：
3.3
作者：
Bettinger, Blaine T.;Clark, Michael G.;Amberg, David C.
通讯作者：
Amberg, David C.
影响因子：
9.2
作者：
Feierbach, B;Chang, F
通讯作者：
Chang, F
影响因子：
3.3
作者：
Conibear, E;Cleck, JN;Stevens, TH
通讯作者：
Stevens, TH
酿酒酵母中SPB重复期间，核心主体（SPB）组件的定位。
影响因子：
7.8
作者：
Adams, I R;Kilmartin, J V
通讯作者：
Kilmartin, J V