Amoeboid T lymphocytes require the septin cytoskeleton for cortical integrity and persistent motility.
Amoeboid T lymphocytes require the septin cytoskeleton for cortical integrity and persistent motility.
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DOI：
10.1038/ncb1808
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发表时间：
2009-01
影响因子：
21.3
通讯作者：
Krummel, Matthew F.
中科院分区：
文献类型：
--
作者：
Tooley, Aaron J.;Gilden, Julia;Jacobelli, Jordan;Beemiller, Peter;Trimble, William S.;Kinoshita, Makoto;Krummel, Matthew F.
在运动过程中细化肌动球蛋白力的系统仍然知之甚少。间隔蛋白聚集在T细胞皮质上，并在中间区富集成细丝。Septin敲除导致膜起泡，过度的前缘突起和尾足的延长。与此相关的刚性丧失允许运动，但细胞变得不协调和持久性差。这也解除了以前未认识到的通过小孔迁移的限制。药理学硬化细胞抵消这种作用，并减轻细胞骨架刚度协同septin消耗。这些数据表明，septins调整肌动球蛋白的力量在运动，并可能调节淋巴细胞运输有限的组织。
The systems that refine actomyosin forces during motility remain poorly understood. Septins assemble on the T cell cortex and are enriched at the mid-zone in filaments. Septin knockdown causes membrane blebbing, excess leading edge protrusions, and lengthening of the trailingedge uropod. The associated loss of rigidity permits motility, but cells become uncoordinated and poorly persistent. This also relieves a previously unrecognized restriction to migration through small pores. Pharmacologically rigidifying cells counteracts this effect, and relieving cytoskeletal rigidity synergizes with septin-depletion. These data suggest that septins tune actomyosin forces during motility, and likely regulate lymphocyte trafficking in confined tissues.
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