Performance of the Luminex xTAG Respiratory Viral Panel Fast in a clinical laboratory setting.
Performance of the Luminex xTAG Respiratory Viral Panel Fast in a clinical laboratory setting.
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DOI：
10.1016/j.jviromet.2012.03.015
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发表时间：
2012-06
影响因子：
3.1
通讯作者：
Lappalainen M
中科院分区：
文献类型：
--
作者：
Jokela P;Piiparinen H;Mannonen L;Auvinen E;Lappalainen M
在临床实验室环境中，RVP Fast检测试剂盒对99.2%的样本给出了有效结果。RVP Fast的检出率上级优于DFA，与荧光定量RT-PCR的检出率相当。RT-RVP Fast比实时RT-PCR检测到更多EV/RV。RVP Fast检测试剂盒需要调整，以涵盖InfA（H1N1）2009病毒。本研究旨在开发用于检测肠道病毒（EV）和鼻病毒（RV）的实时RT-PCR，并评估xTAG RVP Fast检测试剂盒与直接荧光检测试剂盒（DFA）、用于检测呼吸道合胞病毒（RSV）和人偏肺病毒（hMPV）的实时RT-PCR检测试剂盒以及本研究开发的EV/RV RT-PCR检测试剂盒相比的性能。在373份鼻咽样本的分析中评估了RVP Fast检测试剂盒的性能。在2009-2010年收集的297个样本分析中，使用RVP和DFA检测的病毒检出率分别为27.6%和23.8%。这些结果表明RVP Fast检测试剂盒具有统计学显著优效性（P = 0.049）。RVP和RT-PCR对RSV、hMPV、EV和RV的检出率分别为48.0%和45.2%。对于单个靶标，与实时RT-PCR相比，观察到RVP对EV/RV的检出增加（P = 0.043），对甲型流感病毒的检出减少（P = 0.004）。本研究的结果表明，需要调整RVP Fast检测试剂盒的InfA组分，以涵盖InfA（H1N1）2009病毒。
► The RVP Fast assay gave valid results for 99.2% of samples in a clinical laboratory setting. ► The detection rate of RVP Fast was superior to DFA and comparable to real-time RT-PCR. ► RVP Fast detected more EVs/RVs than real-time RT-PCR. ► The RVP Fast assay needs adjustment to also cover the InfA (H1N1)2009 virus. The aim of the study was to develop a real-time RT-PCR for the detection of enteroviruses (EVs) and rhinoviruses (RVs) and to assess the performance of the xTAG RVP Fast assay in comparison to a direct fluorescent assay (DFA), a real-time RT-PCR assay for the detection of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV), and the EV/RV RT-PCR assay developed in this study. The performance of the RVP Fast assay was assessed in the analysis of 373 nasopharyngeal samples. For the viruses of the DFA panel, detection rates of 27.6% and 23.8% were obtained by RVP and DFA, respectively, in analysis of a set of 297 samples collected in 2009–2010. These results show statistically significant superiority of the RVP Fast assay (P = 0.049). For RSV, hMPV, EV, and RV, detection rates of 48.0% and 45.2% were achieved by RVP and RT-PCR, respectively. For individual targets, increased detection of EV/RV (P = 0.043) and decreased detection of influenza A virus (P = 0.004) by RVP in comparison to real-time RT-PCR was observed. The results of the present study imply the need to adjust the InfA component of the RVP Fast assay to also cover the InfA(H1N1) 2009 virus.
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影响因子：
9.4
作者：
Pabbaraju, Kanti;Tokaryk, Kara L.;Fox, Julie D.
通讯作者：
Fox, Julie D.
影响因子：
9.4
作者：
Quan, Phenix-Lan;Palacios, Gustavo;Lipkin, W. Ian
通讯作者：
Lipkin, W. Ian
影响因子：
9.4
作者：
Takahashi, Hiroshi;Norman, Sylvia A.;Patterson, Bruce K.
通讯作者：
Patterson, Bruce K.
DOI：
10.1016/s1386-6532(03)00122-7
发表时间：
2004-03
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Ward CL;Dempsey MH;Ring CJ;Kempson RE;Zhang L;Gor D;Snowden BW;Tisdale M
通讯作者：
Tisdale M
影响因子：
9.4
作者：
Mahony, J.;Chong, S.;Janeczko, R.
通讯作者：
Janeczko, R.