Intrauterine administration of endotoxin leads to motor deficits in a rabbit model: a link between prenatal infection and cerebral palsy.
Intrauterine administration of endotoxin leads to motor deficits in a rabbit model: a link between prenatal infection and cerebral palsy.
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DOI：
10.1016/j.ajog.2008.06.090
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发表时间：
2008-12
影响因子：
9.8
通讯作者：
Chugani, Diane
中科院分区：
文献类型：
--
作者：
Saadani-Makki, Fadoua;Kannan, Sujatha;Lu, Xin;Janisse, James;Dawe, Elizabeth;Edwin, Samuel;Romero, Roberto;Chugani, Diane
确定母体宫内注射内毒素是否会导致新生兔的神经行为缺陷。怀孕的新西兰白兔在妊娠第28/31天向子宫壁注射1ml盐水（n=8）或20μg/kg盐水中的脂多糖（LPS）（n=8）。出生后第 1 天，试剂盒[来自连续 4 窝的盐水 (n=30) 和 LPS (n=18)] 接受了神经行为测试。对新生儿大脑进行小胶质细胞和髓磷脂染色。 LPS组的试剂盒处于高渗状态，并表现出姿势、翻正反射、运动和进食的显着损害，以及由激活的小胶质细胞指示的神经炎症和脑室周围区域的髓鞘形成不足。 LPS 组的死亡率更高（3 窝死产 16 例，1 窝死产 3 例）。母体宫内注射内毒素会导致新生兔的白质损伤和运动缺陷，从而导致类似于脑室周围白质软化和脑瘫的表型。
To determine whether maternal intrauterine endotoxin administration leads to neurobehavioral deficits in newborn rabbits. Pregnant New Zealand white rabbits were injected with 1ml saline (n=8) or 20μg/kg of lipolysaccharide in saline (LPS) (n=8) into the uterine wall on day 28/31 of gestation. On postnatal day 1, kits [saline (n=30) and LPS (n=18) from 4 consecutive litters] underwent neurobehavioral testing. Neonatal brains were stained for microglial cells and myelin. LPS-group kits were hypertonic and demonstrated significant impairment in posture, righting reflex, locomotion and feeding, along with neuroinflammation indicated by activated microglia, and hypomyelination in the periventricular regions. A greater mortality was noted in the LPS group (16 stillbirths from 3 litters vs. 3 from 1 litter). Maternal intrauterine endotoxin administration leads to white matter injury and motor deficits in the newborn rabbit resulting in a phenotype that resembles those found in periventricular leukomalacia and cerebral palsy.
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DOI：
10.1136/fn.88.6.f501
发表时间：
2003-11-01
影响因子：
4.4
作者：
Heep, A;Behrendt, D;Dembinski, J
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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