Estrogen induces distinct patterns of microRNA expression within the mouse uterus.
Estrogen induces distinct patterns of microRNA expression within the mouse uterus.
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DOI：
10.1177/1933719110377472
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Healy C
中科院分区：
文献类型：
--
作者：
Nothnick WB;Healy C
子宫内雌激素活性的控制是显而易见的，因为无对抗性雌激素作用与子宫内膜病变如子宫内膜异位症和子宫内膜癌有关。microRNA已成为重要的转录后调节因子，被认为可以微调类固醇在包括子宫在内的许多系统中的作用。本研究的目的是使用卵巢切除的类固醇重建小鼠模型来检查小鼠子宫内响应于雌激素治疗的microRNA的子宫表达。microRNA微阵列分析和随后的qRT-PCR验证显示，雌激素给药显著增加了mirn 155、mirn 429和mirn 451的表达，而mirn 181 b和mirn 204的表达显著降低。用雌激素受体拮抗剂ICI 182，780预处理证实，雌激素调节通过经典的雌激素受体途径介导。这项研究表明，雌激素调节小鼠子宫内的特定miRNA，可能参与雌激素调节基因的转录后调节。
Control of estrogenic activity within the uterus is evident as unopposed estrogen action is associated with endometrial pathologies such as endometriosis and endometrial carcinoma. MicroRNAs have emerged as important post-transcriptional regulators which are postulated to fine tune the actions of steroids in many systems including the uterus. The objective of the current study was to examine uterine expression of microRNAs in response to estrogen treatment within the mouse uterus using an ovariectomized, steroid reconstituted mouse model. MicroRNA microarray analysis and subsequent qRT-PCR verification revealed that expression of mirn155, mirn429 and mirn451 was significantly increased by estrogen administration while mirn181b and mirn204 expression was significantly reduced. Pretreatment with the estrogen receptor antagonist ICI 182,780 confirmed that estrogen regulation was mediated via the classical estrogen receptor pathway. This study demonstrates that estrogen regulates specific miRNAs within the murine uterus which may participate in post-transcriptional regulation of estrogen-regulated genes.
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影响因子：
4.7
作者：
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通讯作者：
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作者：
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作者：
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作者：
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