Differential expression of microRNAs between eutopic and ectopic endometrium in ovarian endometriosis.
Differential expression of microRNAs between eutopic and ectopic endometrium in ovarian endometriosis.
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DOI：
10.1155/2010/369549
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发表时间：
2010
影响因子：
--
通讯作者：
Surico N
中科院分区：
文献类型：
--
作者：
Filigheddu N;Gregnanin I;Porporato PE;Surico D;Perego B;Galli L;Patrignani C;Graziani A;Surico N
子宫内膜异位症，定义为子宫外存在子宫内膜组织，是一种常见的妇科疾病，发病机制尚不清楚。microrna是一类小的非编码RNA分子的成员，通过抑制靶mrna的翻译在基因表达的转录后调控中起着关键作用。我们通过微阵列分析评估了3例异位子宫内膜与同位子宫内膜中microrna的差异表达。我们鉴定了50个差异表达的microrna，并在其他13例患者中通过实时RT-PCR验证了其中5个microrna的差异表达。我们在计算机上确定了他们的预测靶点，其中一些与基因表达研究中发现的异位子宫内膜与异位子宫内膜差异表达的基因相匹配。对预测靶点的功能分析表明，其中一些靶点涉及子宫内膜异位症的分子途径，从而加强了microrna在该病理中的作用的假设。
Endometriosis, defined as the presence of endometrial tissue outside the uterus, is a common gynecological disease with poorly understood pathogenesis. MicroRNAs are members of a class of small noncoding RNA molecules that have a critical role in posttranscriptional regulation of gene expression by repression of target mRNAs translation. We assessed differentially expressed microRNAs in ectopic endometrium compared with eutopic endometrium in 3 patients through microarray analysis. We identified 50 microRNAs differentially expressed and the differential expression of five microRNAs was validated by real-time RT-PCR in other 13 patients. We identified in silico their predicted targets, several of which match the genes that have been identified to be differentially expressed in ectopic versus eutopic endometrium in studies of gene expression. A functional analysis of the predicted targets indicates that several of these are involved in molecular pathways implicated in endometriosis, thus strengthening the hypothesis of the role of microRNAs in this pathology.
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影响因子：
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作者：
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影响因子：
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