Interleukin-6 is a potent thrombopoietic factor in vivo in mice.
Interleukin-6 is a potent thrombopoietic factor in vivo in mice.
复制标题
Interleukin-6 是小鼠体内有效的血小板生成因子。
作者：
T. Ishibashi;H. Kimura;Y. Shikama;Tatsumi Uchida;S. Kariyone;T. Hirano;T. Kishimoto;F. Takatsuki;Y. Akiyama
为了确定白细胞介素-6（IL-6）对体内巨核细胞生成和血小板生成的生物活性，基于IL-6血清水平的动力学分析，以不同剂量每12小时向小鼠腹膜内施用细胞因子，持续5天或不同的时间间隔，所述动力学分析指示注射后40分钟的峰值，在150分钟时未检测到。剂量反应实验表明，在5微克的平台刺激水平下，IL-6以剂量依赖性方式增加血小板计数。给予5微克IL-6在第5天可重复地使血小板计数增加约50%至60%。此外，巨核细胞的大小响应于IL-6的显着增加引起的治疗，但在巨核细胞的数量没有变化，而IL-6管理没有扩大CFU-MK数。以这种方式进行的体内研究对其他血液学参数的影响可忽略不计，单核细胞水平除外。这些数据表明，IL-6作用于巨核细胞生成的成熟阶段，并促进小鼠体内血小板的产生，表明IL-6具有血小板生成素的功能。
To determine the biologic activity of interleukin-6 (IL-6) on megakaryocytopoiesis and thrombocytopoiesis in vivo, the cytokine was administered intraperitoneally to mice every 12 hours at varying doses for five days or for varying time intervals, based on the kinetic analysis of IL-6 serum levels indicating the peak of 40 minutes following injection, with no detection at 150 minutes. A dose-response experiment showed that IL-6 increased platelet counts in a dose-dependent fashion at a plateau stimulation level of 5 micrograms. Administration of 5 micrograms of IL-6 reproducibly elevated platelet counts at five days by approximately 50% to 60% of increase. Moreover, a striking increase in megakaryocytic size in response to IL-6 was elicited by the treatment, but no change in megakaryocyte numbers; whereas IL-6 administration did not expand CFU-MK numbers. The in vivo studies in this manner had negligible effects on other hematologic parameters, with the minor exception of monocyte levels. These data show that IL-6 acts on maturational stages in megakaryocytopoiesis and promotes platelet production in vivo in mice, suggesting that IL-6 functions as thrombopoietin.
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人重组促红细胞生成素在体外促进小鼠巨核细胞分化。
DOI：
10.1172/jci112796
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Ishibashi,T;Koziol,JA;Burstein,SA
通讯作者：
Burstein,SA
白介素 3 促进分离的单个巨核细胞的分化。
影响因子：
20.3
作者：
Ishibashi,T;Burstein,SA
通讯作者：
Burstein,SA
影响因子：
15.9
作者：
GUERNE, PA;ZURAW, BL;LOTZ, M
通讯作者：
LOTZ, M
DOI：
10.1073/pnas.84.24.9035
发表时间：
1987-12-01
影响因子：
11.1
作者：
IKEBUCHI, K;WONG, GG;OGAWA, M
通讯作者：
OGAWA, M
影响因子：
158.5
作者：
ESCHBACH, JW;EGRIE, JC;ADAMSON, JW
通讯作者：
ADAMSON, JW