Deciphering cellular signals in adult mouse sinoatrial node cells.
Deciphering cellular signals in adult mouse sinoatrial node cells.
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DOI：
10.1016/j.isci.2021.103693
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发表时间：
2022-01-21
期刊：
影响因子：
5.8
通讯作者：
Navedo MF
中科院分区：
文献类型：
--
作者：
Reddy GR;Ren L;Thai PN;Caldwell JL;Zaccolo M;Bossuyt J;Ripplinger CM;Xiang YK;Nieves-Cintrón M;Chiamvimonvat N;Navedo MF
窦房结（SAN）细胞是心脏的起搏器。本研究描述了一种方法，用于培养和感染成年小鼠SAN细胞与FRET为基础的生物传感器，可用于检查信号事件。在含有blebbistatin或（S）-硝基-blebbistatin的培养基中培养的SAN细胞保持其形态、蛋白分布、动作电位（AP）波形和cAMP动力学至少40小时。表达靶向cAMP传感器的SAN细胞显示出不同的β-肾上腺素能介导的cAMP池。SAN细胞中的环GMP、蛋白激酶A、Ca 2 +/CaM激酶II和蛋白激酶D对不同的刺激也表现出独特的动力学。心力衰竭SAN细胞显示cAMP和cGMP水平降低。总之，一个可靠的方法来维持成年小鼠SAN细胞在文化中，这有利于在生理和病理条件下的信号网络和调节机制的研究。来自健康和疾病状态小鼠的成年SAN细胞的优化培养方法培养的成年SAN细胞可以用不同的荧光生物传感器感染成年SAN细胞中的信号网络的检查，包括离散的cAMP池，SAN细胞cAMP和cGMP信号在心力衰竭期间受损细胞生物学;细胞生物学的功能方面;生物学实验方法
Sinoatrial node (SAN) cells are the pacemakers of the heart. This study describes a method for culturing and infection of adult mouse SAN cells with FRET-based biosensors that can be exploited to examine signaling events. SAN cells cultured in media with blebbistatin or (S)-nitro-blebbistatin retain their morphology, protein distribution, action potential (AP) waveform, and cAMP dynamics for at least 40 h. SAN cells expressing targeted cAMP sensors show distinct β-adrenergic-mediated cAMP pools. Cyclic GMP, protein kinase A, Ca2+/CaM kinase II, and protein kinase D in SAN cells also show unique dynamics to different stimuli. Heart failure SAN cells show a decrease in cAMP and cGMP levels. In summary, a reliable method for maintaining adult mouse SAN cells in culture is presented, which facilitates studies of signaling networks and regulatory mechanisms during physiological and pathological conditions. Optimized culturing method of adult SAN cells from healthy and disease-state mice Cultured adult SAN cells can be infected with diverse fluorescence biosensors Examination of signaling networks, including discrete cAMP pools, in adult SAN cells SAN cells cAMP and cGMP signals are impaired during heart failure Cell biology; Functional aspects of cell biology; Biology experimental methods
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DOI：
10.1016/j.bbrc.2004.06.045
发表时间：
2004-07-30
影响因子：
3.1
作者：
Kolega, J
通讯作者：
Kolega, J
影响因子：
14.8
作者：
Luo, Jinyong;Deng, Zhong-Liang;He, Tong-Chuan
通讯作者：
He, Tong-Chuan
影响因子：
14.8
作者：
Fenske, Stefanie;Proebstle, Rasmus;Wahl-Schott, Christian
通讯作者：
Wahl-Schott, Christian
影响因子：
10.8
作者：
Mangoni, ME;Nargeot, J
通讯作者：
Nargeot, J
影响因子：
16.6
作者：
Kepiro, Miklos;Varkuti, Boglarka H.;Malnasi-Csizmadia, Andras
通讯作者：
Malnasi-Csizmadia, Andras