Diversity of Immunoglobulin Light Chain Genes in Non-Teleost Ray-Finned Fish Uncovers IgL Subdivision into Five Ancient Isotypes.
Diversity of Immunoglobulin Light Chain Genes in Non-Teleost Ray-Finned Fish Uncovers IgL Subdivision into Five Ancient Isotypes.
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DOI：
10.3389/fimmu.2018.01079
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发表时间：
2018
影响因子：
7.3
通讯作者：
Taranin AV
中科院分区：
文献类型：
--
作者：
Guselnikov SV;Baranov KO;Najakshin AM;Mechetina LV;Chikaev NA;Makunin AI;Kulemzin SV;Andreyushkova DA;Stöck M;Wuertz S;Gessner J;Warren WC;Schartl M;Trifonov VA;Taranin AV
这项研究的目的是通过研究非硬骨鱼IGL基因的结构和多样性来填补免疫球蛋白进化史上的重要空白。首先，基于对最近的转录和基因组资源的生物信息学分析，我们对软骨鱼(Acipenser ruthenus(Sterlet))的IGL基因进行了实验表征。我们发现该物种有三个编码IGL kappa样链的基因座和一个单一的VJC簇，编码均一的lambda样轻链。此外，Strlet还具有Sigma样的VL和J-CL基因，这两个基因分别转录，并编码带有可切割的前导肽的蛋白质产物。AcipenSerformes IGL数据集通过在属于其他非硬骨鱼类谱系的物种数据库中挖掘的序列来扩展：全鳍和多翅目鱼类。将这些新数据纳入系统发育分析，表明IGL链被明确地细分为五个组。之前被描述为硬骨鱼IGL lambda的同种类型后来被证明是在射线鳍鱼辐射之前出现的kappa和lambda链副对数。我们将其命名为lambda-2。系统发育还表明，最初被认为是软骨鱼类特有的sigma-2 IGL链存在于海龙、多翅目动物，甚至在海龟中。我们得出结论，古代有五种IGL亚型，它们在颌骨脊椎动物的不同谱系中进化不同。
The aim of this study was to fill important gaps in the evolutionary history of immunoglobulins by examining the structure and diversity of IgL genes in non-teleost ray-finned fish. First, based on the bioinformatic analysis of recent transcriptomic and genomic resources, we experimentally characterized the IgL genes in the chondrostean fish, Acipenser ruthenus (sterlet). We show that this species has three loci encoding IgL kappa-like chains with a translocon-type gene organization and a single VJC cluster, encoding homogeneous lambda-like light chain. In addition, sterlet possesses sigma-like VL and J-CL genes, which are transcribed separately and both encode protein products with cleavable leader peptides. The Acipenseriformes IgL dataset was extended by the sequences mined in the databases of species belonging to other non-teleost lineages of ray-finned fish: Holostei and Polypteriformes. Inclusion of these new data into phylogenetic analysis showed a clear subdivision of IgL chains into five groups. The isotype described previously as the teleostean IgL lambda turned out to be a kappa and lambda chain paralog that emerged before the radiation of ray-finned fish. We designate this isotype as lambda-2. The phylogeny also showed that sigma-2 IgL chains initially regarded as specific for cartilaginous fish are present in holosteans, polypterids, and even in turtles. We conclude that there were five ancient IgL isotypes, which evolved differentially in various lineages of jawed vertebrates.
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影响因子：
3.2
作者：
Edholm, Eva-Stina;Wilson, Melanie;Bengten, Eva
通讯作者：
Bengten, Eva
影响因子：
7
作者：
Li, Ruiqiang;Zhu, Hongmei;Wang, Jun
通讯作者：
Wang, Jun
DOI：
10.1084/jem.182.1.109
发表时间：
1995-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Anderson MK;Shamblott MJ;Litman RT;Litman GW
通讯作者：
Litman GW
影响因子：
1.8
作者：
Edholm, Eva-Stina;Hudgens, Edward D.;Wilson, Melanie
通讯作者：
Wilson, Melanie
影响因子：
3.7
作者：
Huang T;Zhang M;Wei Z;Wang P;Sun Y;Hu X;Ren L;Meng Q;Zhang R;Guo Y;Hammarstrom L;Li N;Zhao Y
通讯作者：
Zhao Y