Does the cell number of 0PN embryos on day 3 affect pregnancy and neonatal outcomes following single blastocyst transfer?
Does the cell number of 0PN embryos on day 3 affect pregnancy and neonatal outcomes following single blastocyst transfer?
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第 3 天 0PN 胚胎的细胞数量是否会影响单囊胚移植后的妊娠和新生儿结局？
DOI：
10.1186/s12884-022-04492-7
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发表时间：
2022-03-12
影响因子：
3.1
通讯作者：
Lyu Q
中科院分区：
文献类型：
--
作者：
Chen C;Li W;Yin M;Li M;Wu L;Si J;Zhao L;Li B;Yan Z;Lyu Q
0 PN受精卵的卵裂率低，卵裂期胚胎移植的临床结果不令人满意。囊胚培养用于筛选0 PN胚胎，但第3天0 PN胚胎的细胞数是否影响单囊胚移植后的临床结局尚不清楚，这将有助于评估这些胚胎的临床价值。这项回顾性研究比较了2015年1月至2019年10月在一家三级医疗学术医疗中心进行的46，804例0 PN受精卵、242例0 PN冻融单囊胚移植和92例相应的0 PN单胎与232，441例2 PN受精卵、3563例2 PN冻融单囊胚移植和1250例2 PN单胎。将0 PN和2 PN胚胎分为两组：第3天细胞数< 6组和细胞数≥ 6组。比较两组胚胎发育、妊娠结局及新生儿结局。0 PN受精卵的卵裂率和可用囊胚率均明显低于2 PN受精卵（25.9%vs.97.4%，P < 0.001; 13.9%vs.23.4%，P < 0.001）。在< 6细胞组中，0 PN胚胎的可用囊胚率显著低于2 PN胚胎（2.5% vs.12.7%，P < 0.001）。但在≥ 6细胞组中，0 PN卵裂胚胎的可用囊胚率显著提高，但略低于2 PN胚胎（33.9%vs.35.7%，P = 0.014）。重要的是，与2 PN单囊胚移植相比，0 PN单囊胚移植的临床妊娠率、活产率、Z评分和畸形率在< 6细胞组中均无显著差异（30.4% vs. 39.8%，P = 0.362; 30.4% vs. 31.3%，P = 0.932; 0.89 ± 0.90 vs. 0.42 ± 1.02，P = 0.161; 0% vs. 2.6%，P = 1.000）或≥ 6个细胞组（50.7%对比46.6%，P = 0.246; 39.7%对比38.3%，P = 0.677; 0.50 ± 1.23对比0.47 ± 1.11，P = 0.861; 2.4%对比1.8%，P = 1.000）。0 PN胚胎第3天的细胞数影响随后的囊胚形成，但不影响0 PN单囊胚移植的随后妊娠和新生儿结局，这可能有利于临床医生就0 PN胚胎的临床价值向患者提供咨询。在线版本包含补充材料，可通过10.1186/s12884-022-04492-7获得。
0PN zygotes have a low cleavage rate, and the clinical outcomes of cleavage-stage embryo transfers are unsatisfactory. Blastocyst culturing is used to screen 0PN embryos, but whether the cell number of 0PN embryos on day 3 affects the clinical outcomes following single blastocyst transfer is unknown and would be helpful in evaluating the clinical value of these embryos. This retrospective study compared 46,804 0PN zygotes, 242 0PN frozen-thawed single blastocyst transfers, and 92 corresponding 0PN singletons with 232,441 2PN zygotes, 3563 2PN frozen-thawed single blastocyst transfers, and 1250 2PN singletons from January 2015 to October 2019 at a tertiary-care academic medical centre. The 0PN and 2PN embryos were divided into two groups: the group with < 6 cells on day 3 and that with ≥ 6 cells. Embryo development, subsequent pregnancy and neonatal outcomes were compared between the two groups. The cleavage and available blastocyst rates of the 0PN zygotes were much lower than those of the 2PN zygotes (25.9% vs. 97.4%, P < 0.001; 13.9% vs. 23.4%, P < 0.001). In the < 6 cells group, the available blastocyst rate of the cleaved 0PN embryos was significantly lower than that of the 2PN embryos (2.5% vs. 12.7%, P < 0.001). However, in the ≥ 6 cells group, the available blastocyst rate of the 0PN cleaved embryos significantly improved, although it was slightly lower than that of the 2PN embryos (33.9% vs. 35.7%, P = 0.014). Importantly, compared to those of the 2PN single blastocyst transfers, the clinical pregnancy rate, live birth rate, Z-score and malformation rate of the 0PN single blastocyst transfers were not significantly different in either the < 6 cells group (30.4% vs. 39.8%, P = 0.362; 30.4% vs. 31.3%, P = 0.932; 0.89 ± 0.90 vs. 0.42 ± 1.02, P = 0.161; 0% vs. 2.6%, P = 1.000) or the ≥ 6 cells group (50.7% vs. 46.6%, P = 0.246; 39.7% vs. 38.3%, P = 0.677; 0.50 ± 1.23 vs. 0.47 ± 1.11, P = 0.861; 2.4% vs. 1.8%, P = 1.000). The cell number on day 3 of 0PN embryos affected the subsequent formation of blastocysts but did not influence the subsequent pregnancy and neonatal outcomes of 0PN single blastocyst transfers, which may be beneficial to clinicians counselling patients on the clinical value of 0PN embryos. The online version contains supplementary material available at 10.1186/s12884-022-04492-7.
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影响因子：
4
作者：
Chen X;Shi S;Mao J;Zou L;Yu K
通讯作者：
Yu K
影响因子：
3.9
作者：
Baran J;Weres A;Czenczek-Lewandowska E;Leszczak J;Kalandyk-Osinko K;Łuszczki E;Sobek G;Mazur A
通讯作者：
Mazur A
DOI：
10.1080/14647270600636269
发表时间：
2006-09-01
期刊：
Human fertility (Cambridge, England)
影响因子：
--
作者：
Feenan, Katie;Herbert, Mary
通讯作者：
Herbert, Mary
影响因子：
2.9
作者：
Ji, Hui;Zhou, Yuxi;Shen, Rong
通讯作者：
Shen, Rong
影响因子：
3.4
作者：
Hondo, Saki;Arichi, Akane;Shimizu, Yasufumi
通讯作者：
Shimizu, Yasufumi