Iron–Sulfur Cluster Biosynthesis in Algae with Complex Plastids
Iron–Sulfur Cluster Biosynthesis in Algae with Complex Plastids
复制标题
具有复杂质体的藻类中铁硫簇的生物合成
DOI：
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发表时间：
2018
影响因子：
3.3
通讯作者：
U. Maier
中科院分区：
文献类型：
--
作者：
Christopher Grosche;A. Diehl;S. Rensing;U. Maier
摘要质体由四层膜包围，在内外质体膜之间有一个特殊的隔室，称为质周隔室（PPC）。这种细胞结构通常被认为是真核光合内共生体的细胞质，它被整合到宿主细胞中，并被流线化成具有复杂膜结构的质体。到目前为止，在任何代表的PPC中均未鉴定出细胞器或细胞器相关的细胞器。然而，两个突出的群体，隐藻和chlorarachniophytes，仍然怀有一个减少的细胞核共生体的起源，核形，在他们的PPC。通常，许多位于细胞质和细胞核的真核蛋白质需要铁-硫辅因子来实现其功能。除了一些例外，它们的合成取决于位于南极洲的所谓铁硫复合物（ISC）组装机器。这种机制为细胞质提供了一种未知的硫成分，然后通过细胞质铁硫蛋白组装（CIA）机制将其转化为铁硫簇。在这里，我们调查了CIA的机器是否存在于缺乏质子的PPC中。通过使用生物信息学筛选和候选蛋白的体内定位，我们表明PPC特异性CIA机器的存在与核态的存在相关。PPC和主机特定的CIA组件的系统发育分析还表明了一个复杂的进化的CIA机器在生物体中的质体包围四个膜。
Abstract Plastids surrounded by four membranes harbor a special compartment between the outer and inner plastid membrane pair, the so-called periplastidal compartment (PPC). This cellular structure is usually presumed to be the reduced cytoplasm of a eukaryotic phototrophic endosymbiont, which was integrated into a host cell and streamlined into a plastid with a complex membrane structure. Up to date, no mitochondrion or mitochondrion-related organelle has been identified in the PPC of any representative. However, two prominent groups, the cryptophytes and the chlorarachniophytes, still harbor a reduced cell nucleus of symbiont origin, the nucleomorph, in their PPCs. Generally, many cytoplasmic and nucleus-located eukaryotic proteins need an iron–sulfur cofactor for their functionality. Beside some exceptions, their synthesis is depending on a so-called iron–sulfur complex (ISC) assembly machinery located in the mitochondrion. This machinery provides the cytoplasm with a still unknown sulfur component, which is then converted into iron–sulfur clusters via a cytosolic iron–sulfur protein assembly (CIA) machinery. Here, we investigated if a CIA machinery is present in mitochondrion-lacking PPCs. By using bioinformatic screens and in vivo-localizations of candidate proteins, we show that the presence of a PPC-specific CIA machinery correlates with the presence of a nucleomorph. Phylogenetic analyses of PPC- and host specific CIA components additionally indicate a complex evolution of the CIA machineries in organisms having plastids surrounded by four membranes.
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DOI：
10.1126/science.1219723
发表时间：
2012-07-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Stehling O;Vashisht AA;Mascarenhas J;Jonsson ZO;Sharma T;Netz DJ;Pierik AJ;Wohlschlegel JA;Lill R
通讯作者：
Lill R
影响因子：
6.6
作者：
Lill, Roland;Dutkiewicz, Rafal;Milhlenhoff, Ulrich
通讯作者：
Milhlenhoff, Ulrich
影响因子：
3.3
作者：
Petersen J;Ludewig AK;Michael V;Bunk B;Jarek M;Baurain D;Brinkmann H
通讯作者：
Brinkmann H
影响因子：
3.3
作者：
Grosche C;Funk HT;Maier UG;Zauner S
通讯作者：
Zauner S
影响因子：
29
作者：
Stehling O;Mascarenhas J;Vashisht AA;Sheftel AD;Niggemeyer B;Rösser R;Pierik AJ;Wohlschlegel JA;Lill R
通讯作者：
Lill R