Chromera velia, endosymbioses and the rhodoplex hypothesis--plastid evolution in cryptophytes, alveolates, stramenopiles, and haptophytes (CASH lineages).
Chromera velia, endosymbioses and the rhodoplex hypothesis--plastid evolution in cryptophytes, alveolates, stramenopiles, and haptophytes (CASH lineages).
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DOI：
10.1093/gbe/evu043
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发表时间：
2014-03
影响因子：
3.3
通讯作者：
Brinkmann H
中科院分区：
文献类型：
--
作者：
Petersen J;Ludewig AK;Michael V;Bunk B;Jarek M;Baurain D;Brinkmann H
Chromera Velia的发现为研究疟疾寄生虫的藻类祖先提供了一个意想不到的机会。Chromera Velia是顶端复合体病原体的自由生活的光合作用近亲。在这项工作中，我们比较了真核生物到真核生物在C.Velia中内共生的分子足迹，以及它们在含有周围素的甲藻(PCD)中的等价物，以重新评估最近支持它们的叶绿体共同祖先的说法。为此，我们通过下一代测序建立了毛囊线虫的基因组草稿和一套全长的cDNAs序列。我们记录了线粒体基因组中只有一个Coxi基因，因此它代表了迄今为止发现的基因最少的需氧细胞器，但我们的分析集中在Calvin循环的五个“幸运基因”上。它们之所以被选中，是因为它们通过与红藻的单一次级内生共生，支持来自隐藻、泡状体(以PCD为代表)、稻壳藻和触藻(CASH)的复杂质体的共同起源。正如预期的那样，我们对核编码的Calvin循环标记的广泛采样的系统发育支持Chromera的复杂质体的视觉起源。然而，他们也提出通过两个真核生物到真核生物的内生生物来独立地起源顶端复合体和恐藻体(PCD)。尽管这一结论与当前关于泡状藻共同的光合作用祖先的观点不一致，但这一结论与脊鞭毛虫的异常质体结构以及各自谱系中四个与三个叶绿体膜的形态悖论是一致的。通过对另外五个标记的分析，进一步支持了独立的内生生物，其中四个标记参与了叶绿体蛋白的输入机制。最后，我们引入了“Roudoplex假说”作为一种方便的方法来指定进化场景，其中现金叶绿体最终是与红藻的单个次级内共生的产物，但随后通过更高阶的真核生物到真核生物的内共生水平传播。
The discovery of Chromera velia, a free-living photosynthetic relative of apicomplexan pathogens, has provided an unexpected opportunity to study the algal ancestry of malaria parasites. In this work, we compared the molecular footprints of a eukaryote-to-eukaryote endosymbiosis in C. velia to their equivalents in peridinin-containing dinoflagellates (PCD) to reevaluate recent claims in favor of a common ancestry of their plastids. To this end, we established the draft genome and a set of full-length cDNA sequences from C. velia via next-generation sequencing. We documented the presence of a single coxI gene in the mitochondrial genome, which thus represents the genetically most reduced aerobic organelle identified so far, but focused our analyses on five “lucky genes” of the Calvin cycle. These were selected because of their known support for a common origin of complex plastids from cryptophytes, alveolates (represented by PCDs), stramenopiles, and haptophytes (CASH) via a single secondary endosymbiosis with a red alga. As expected, our broadly sampled phylogenies of the nuclear-encoded Calvin cycle markers support a rhodophycean origin for the complex plastid of Chromera. However, they also suggest an independent origin of apicomplexan and dinophycean (PCD) plastids via two eukaryote-to-eukaryote endosymbioses. Although at odds with the current view of a common photosynthetic ancestry for alveolates, this conclusion is nonetheless in line with the deviant plastome architecture in dinoflagellates and the morphological paradox of four versus three plastid membranes in the respective lineages. Further support for independent endosymbioses is provided by analysis of five additional markers, four of them involved in the plastid protein import machinery. Finally, we introduce the “rhodoplex hypothesis” as a convenient way to designate evolutionary scenarios where CASH plastids are ultimately the product of a single secondary endosymbiosis with a red alga but were subsequently horizontally spread via higher-order eukaryote-to-eukaryote endosymbioses.
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影响因子：
64.5
作者：
Clasquin MF;Melamud E;Singer A;Gooding JR;Xu X;Dong A;Cui H;Campagna SR;Savchenko A;Yakunin AF;Rabinowitz JD;Caudy AA
通讯作者：
Caudy AA
具有复杂红色质体的生物体中的ERAD成分表明，募集了Periplastid膜的蛋白质转运途径。
影响因子：
3.3
作者：
Felsner G;Sommer MS;Gruenheit N;Hempel F;Moog D;Zauner S;Martin W;Maier UG
通讯作者：
Maier UG
影响因子：
3.3
作者：
Deschamps P;Moreira D
通讯作者：
Moreira D
影响因子：
2.2
作者：
Adl, SM;Simpson, AGB;Taylor, MFJR
通讯作者：
Taylor, MFJR
影响因子：
3.3
作者：
Burki, Fabien;Shalchian-Tabrizi, Kamran;Pawlowski, Jan
通讯作者：
Pawlowski, Jan