Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass.
Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass.
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围手术期血浆线粒体 DNA 的变化与体外循环心脏手术引起的炎症细胞因子峰值相关
DOI：
10.1186/s13019-015-0298-6
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发表时间：
2015-06-24
影响因子：
1.6
通讯作者：
Meng W
中科院分区：
文献类型：
--
作者：
Qin C;Liu R;Gu J;Li Y;Qian H;Shi Y;Meng W
体外循环（CPB）心脏手术可能引起炎症反应，这可能会恶化结局。肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、-8和-10等炎性细胞因子可作为术后炎症反应的效应因子和预测因子。血浆线粒体DNA（mtDNA）是近年来发现的一种促炎因子，当细胞受到损伤时会释放出来。在本研究中，我们纳入了38例接受冠状动脉旁路移植术（CABG）的患者，分析他们围手术期血浆mtDNA和炎症细胞因子水平。分别在主动脉阻断前（T1）、体外循环结束时（T2）、体外循环后6小时（T3）、体外循环后12小时（T4）和体外循环后24小时（T5）采集血样。采用RT-PCR和ELISA试剂盒分别检测血浆线粒体DNA和炎性细胞因子。采用双变量相关分析分别检测血浆mtDNA与炎症因子的相关性。结果表明，血浆mtDNA在T2时显著升高，在T4时达到峰值。此外，血浆TNF-α、IL-6和IL-8水平在T2时显著升高，在T3时达到峰值，而IL-10升高，在T2时达到峰值。双变量相关分析显示，血浆mtDNA峰值与TNF-α峰值（r = 0.677，P < 0.001）、IL-6峰值（r = 0.706，P < 0.001）、IL-8峰值（r = 0.584，P < 0.001）和IL-10峰值（r = 0.565，P < 0.001）呈正相关。我们发现血浆mtDNA可能在CPB诱导的术后炎症反应中起关键作用。
Cardiac surgery with cardiopulmonary bypass (CPB) may cause inflammatory responses, which can deteriorate the outcomes. Inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6,–8 and -10, can act as both the effector and the predictor for post-operative inflammatory responses. Plasma mitochondrial DNA (mtDNA) was found as a pro-inflammatory agent recently, which was released when cells were insulted. In the present study, we included 38 patients undergoing coronary artery bypass graft (CABG) to analyze their perioperative plasma mtDNA and levels of inflammatory cytokines. Blood samples were collected before aortic cross-clamping (T1), at the end of CPB (T2), 6 h post-CPB (T3), 12 h post-CPB (T4), and 24 h post-CPB (T5). Rt-PCR and specific ELISA kits were used to quantify the plasma mtDNA and inflammatory cytokines, respectively. Bivariate correlations analysis was used to check the correlations between plasma mtDNA and inflammatory cytokines respectively. Results shown that plasma mtDNA elevated significantly at T2 and peaked at T4. Furthermore, plasma TNF-α, IL-6 and IL-8 levels significantly increased at T2 and peaked at T3 while IL-10 elevated and peaked at T2. Bivariate correlations analysis showed that the peak plasma mtDNA were positively correlated with the peak TNF-α (r = 0.677, P < 0.001), the peak IL-6 (r = 0.706, P < 0.001), the peak IL-8 (r = 0.584, P < 0.001) and the peak IL-10 (r = 0.565, P < 0.001). We found that plasma mtDNA might play a key role in CPB-induced post-operative inflammatory responses.
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