The genomes of a monogenic fly: views of primitive sex chromosomes.

The genomes of a monogenic fly: views of primitive sex chromosomes.
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DOI:
10.1038/s41598-020-72880-0
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发表时间:
2020-09-25
期刊:
影响因子:
4.6
通讯作者:
Picard CJ
Picard CJ
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Andere AA;Pimsler ML;Tarone AM;Picard CJ

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雄性和雌性后代的产生通常由控制性别特异性表达的特定性染色体的存在决定,性染色体通过减少重组和专门的基因内容进化。在这里,我们提出的基因组的红腹果蝇,单基因的打击苍蝇(女性产生女性或男性后代,专门)通过单独测序和组装每种类型的女性和男性。基因组(> 25倍覆盖率)似乎没有任何性连锁的Muller F元件(许多双翅目的典型元件),并且在支持C.红褐色同形染色体。在这个物种中的男性与单峰覆盖分布,而女性表现出双峰覆盖分布,这表明在基因组结构的潜在差异。个体性别草案基因组的存在提供了新的线索,关于双翅目内观察到的不同性别决定机制的起源和进化。性染色体和性别决定基因的其他打击苍蝇的额外的基因组分析将允许一个完善的进化理解如何苍蝇与一个典型的X/Y异配子生殖(男性和女性后代在类似的比例)性别决定系统进化成一个具有主导因素,导致在染色体单态系统中的单一性别后代。
The production of male and female offspring is often determined by the presence of specific sex chromosomes which control sex-specific expression, and sex chromosomes evolve through reduced recombination and specialized gene content. Here we present the genomes of Chrysomya rufifacies, a monogenic blow fly (females produce female or male offspring, exclusively) by separately sequencing and assembling each type of female and the male. The genomes (> 25X coverage) do not appear to have any sex-linked Muller F elements (typical for many Diptera) and exhibit little differentiation between groups supporting the morphological assessments of C. rufifacies homomorphic chromosomes. Males in this species are associated with a unimodal coverage distribution while females exhibit bimodal coverage distributions, suggesting a potential difference in genomic architecture. The presence of the individual-sex draft genomes herein provides new clues regarding the origination and evolution of the diverse sex-determining mechanisms observed within Diptera. Additional genomic analysis of sex chromosomes and sex-determining genes of other blow flies will allow a refined evolutionary understanding of how flies with a typical X/Y heterogametic amphogeny (male and female offspring in similar ratios) sex determination systems evolved into one with a dominant factor that results in single sex progeny in a chromosomally monomorphic system.
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