Orphanin FQ, but not dynorphin A, accelerates colonic transit in rats.
Orphanin FQ, but not dynorphin A, accelerates colonic transit in rats.
复制标题
孤啡肽 FQ（而非强啡肽 A）可加速大鼠的结肠转运。
DOI：
10.1053/gast.2000.8557
复制
发表时间：
2000
期刊：
影响因子：
29.4
通讯作者：
Owyang,C
中科院分区：
文献类型：
--
作者：
Takahashi,T;Mizuta,Y;Owyang,C
背景与目的内源性阿片受体样1配体--强啡肽FQ（OFQ），结构上与强啡肽A相似。我们研究了孤啡肽在结肠中的作用机制，并与强啡肽A在vivo.MethodsColonic contractions通过微型力传感器记录在大鼠结肠serenum表面植入。结果孤啡肽（0.01-3 nmol/kg）引起大鼠结肠收缩，在剂量依赖性的方式。孤啡肽诱导的结肠收缩不受外源性去神经支配的影响，但被河豚毒素消除。连续输注OFQ（1 nmol · kg-1· min-1）和强啡肽A（100 nmol · kg-1· min-1）在近端结肠诱导相似的时相收缩。然而，由2种肽诱导的收缩活性在中段和末端结肠中显著不同。由孤啡肽输注引起的巨大收缩从结肠中段迁移到远端。相反，强啡肽A引起整个结肠的同时收缩，并没有流产迁移。皮下注射孤啡肽（1-3 nmol/kg）可促进大鼠结肠运输，而强啡肽A（30-100 nmol/kg）可延迟大鼠结肠运输。
Background & AimsThe endogenous opiate receptor-like 1 ligand, orphanin FQ (OFQ), which structurally resembles dynorphin A, has been identified. We investigated the mechanism of action of OFQ in the colon and compared it with that of dynorphin A in vivo.MethodsColonic contractions were recorded via miniature force transducers implanted on the serosal surface of the rat colon.ResultsIntravenous administration of OFQ (0.01–3 nmol/kg) induced contractions in the rat colon in a dose-dependent manner. Colonic contractions induced by OFQ were not affected by extrinsic denervation but abolished by tetrodotoxin. Continuous infusion of OFQ (1 nmol · kg−1· min−1) and dynorphin A (100 nmol · kg−1· min−1) induced similar phasic contractions in the proximal colon. However, the contractile activity induced by the 2 peptides differed significantly in the mid and distal colon. Giant contractions induced by OFQ infusion migrated from the mid to distal colon. In contrast, dynorphin A evoked simultaneous contractions throughout the entire colon, which did not migrate aborally. Subcutaneous administration of OFQ (1–3 nmol/kg) accelerated colonic transit, whereas dynorphin A (30–100 nmol/kg) delayed colonic transit.ConclusionsThe results show that OFQ accelerates colonic transit by promoting migrating colonic contractions in rats.
登录
查看更多内容
伤害感受素受体的药理学特征：体外研究。
影响因子：
2.1
作者：
G. Calo’;A. Rizzi;M. Bodin;W. Neugebauer;S. Salvadori;R. Guerrini;C. Bianchi;D. Regoli
通讯作者：
D. Regoli
DOI：
10.1152/ajpgi.1999.276.1.g125
发表时间：
1999
期刊：
American journal of physiology. Gastrointestinal and liver physiology
影响因子：
--
作者：
M. Osinski;Paul Bass;E. Gaumnitz
通讯作者：
M. Osinski;Paul Bass;E. Gaumnitz
一氧化氮合成酶抑制剂可预防大鼠蓖麻油引起的腹泻
影响因子：
7.3
作者：
N. Mascolo;A. Izzo;F. Barbato;F. Capasso
通讯作者：
F. Capasso
新型阿片类药物伤害感受素对支配豚鼠气管的副交感神经释放乙酰胆碱的纳洛酮不敏感抑制
影响因子：
7.3
作者：
H. Patel;M. Giembycz;L. Spicuzza;P. J. Barnes;M. Belvisi
通讯作者：
M. Belvisi
影响因子：
4.8
作者：
Reinscheid, RK;Higelin, J;Civelli, O
通讯作者：
Civelli, O