Temperature affects the biology of Schmidtea mediterranea.

Temperature affects the biology of Schmidtea mediterranea.
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DOI:
10.1038/s41598-018-33355-5
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发表时间:
2018-10-08
期刊:
影响因子:
4.6
通讯作者:
Drancourt M
Drancourt M
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Hammoudi N;Torre C;Ghigo E;Drancourt M

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利用地中海施米德(Schmidtea mediterranea)模型涡虫在19°C的实验温度下进行了组织再生和宿主-病原体相互作用的研究。观察暴露于19°C - 32°C环境下的地中海拟涡虫3个月的存活、移动、摄食和再生情况,以及6天内金黄色葡萄球菌病原体的消除情况。观察18天后,地中海拟涡虫在30°C - 32°C的温度下死亡,但在19°C至28°C的温度下耐受,流动性和摄食行为无显著差异。RNAi饲喂测试的遗传延展性在26°C和28°C时仍然有效。关于涡虫的免疫能力,我们报道了被金黄色葡萄球菌感染的蠕虫在26°C和28°C时的免疫反应加剧。这些观察结果提示涡虫干细胞的温度调节,并说明在使用涡虫作为模式生物研究再生和免疫反应时调节实验温度的重要性。
Studies of tissue regeneration and host-pathogen interactions using the model planarian Schmidtea mediterranea have been performed at an experimental temperature of 19 °C. S. mediterranea planarians exposed to 19 °C–32 °C were observed for survival, mobility, feeding and regeneration for three months and elimination of the Staphylococcus aureus pathogen over six days. S. mediterranea planarians died at 30 °C–32 °C after 18 days of observation but tolerated temperatures of 19 °C up to 28 °C with non-significant differences in mobility and feeding behavior. Genetic malleability tested by RNAi feeding was still efficient at 26 °C and 28 °C. Concerning the immune capacity of planarians, we reported an exacerbation of the immune response in worms infected by S. aureus at 26 °C and 28 °C. These observations suggest a temperature modulation of planarian stem cells and illustrate the importance of modulating experimental temperature when using planarians as model organisms to study regeneration and immune response.
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