Interplay between bladder microbiota and overactive bladder symptom severity: a cross-sectional study.
Interplay between bladder microbiota and overactive bladder symptom severity: a cross-sectional study.
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膀胱微生物群与膀胱过度活动症症状严重程度之间的相互作用：一项横断面研究
DOI：
10.1186/s12894-022-00990-0
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发表时间：
2022-03-19
期刊：
影响因子：
2
通讯作者：
Wu P
中科院分区：
文献类型：
--
作者：
Li K;Chen C;Zeng J;Wen Y;Chen W;Zhao J;Wu P
健康人的泌尿道中存在着微生物群，这一点已被广泛接受。泌尿微生物组的不平衡在各种良性泌尿系统疾病的发展中起着重要作用，包括下尿路症状（LUTS）和膀胱过度活动症（OAB）。然而，尿微生物组的改变是否对OAB症状的严重程度产生影响尚未阐明。本研究的目的是检查尿液微生物组与OAB严重程度之间的相关性。对70例OAB患者进行膀胱过度活动症症状评分（OABSS）问卷调查。获得导管尿液样本用于16 S rRNA基因测序。通过α多样性评估物种丰富度和均匀度，通过β多样性评估轻度或中度/重度严重程度患者之间的尿液微生物组差异。使用Pearson相关分析评估尿微生物组与OAB症状严重程度之间的关系。轻度患者（OABSS ≤ 5，n = 17）的细菌多样性（Simpson指数，P = 0.024）和丰富度（Chao 1，P = 0.023）低于中重度患者（OABSS > 5，n = 53）。两组尿微生物β多样性差异有统计学意义。OABSS评分与尿微生物丰富度指数（Chao 1，P = 0.002）和多样性指数（Shannon指数，P = 0.044）呈正相关。某些细菌属（例如，卟啉单胞菌和普雷沃菌）与OAB亚症状的严重程度显著相关。本研究表明，尿液微生物组与OAB症状的严重程度密切相关，尿液微生物组的多样性和丰富性增加伴随着更严重的OAB症状，表明尿液微生态失调可能在功能性膀胱疾病的恶化中起关键作用。
It is widely accepted that there exist microbiota communities in urinary tract of healthy individuals. Imbalance in the urinary microbiome plays important roles in the development of various benign urological conditions including lower urinary track symptoms (LUTS) and overactive bladder (OAB). However, whether alteration in urinary microbiome exerts influence on the severity of OAB symptom has yet to be elucidated. The purpose of this study is to examine the correlation between urinary microbiome and the severity of OAB. A total of 70 OAB patients were recruited to finish overactive bladder symptom score (OABSS) questionnaires. Catheterized urine samples were obtained for 16S rRNA gene sequencing. The species richness and evenness were evaluated by α diversity, and the difference in urinary microbiome between patients with mild or moderate/severe severity was evaluated by β diversity. The relationship between urinary microbiome and the severity of OAB symptom was evaluated using Pearson’s correlation analysis. Mild patients (OABSS ≤ 5, n = 17) had lower bacterial diversity (Simpson index, P = 0.024) and richness (Chao1, P = 0.023) than those with moderate/severe symptom (OABSS > 5, n = 53). Beta-diversity of urinary microbiome between two groups were significantly different. Furthermore, the score of OABSS was positively correlated with the richness index (Chao1, P = 0.002) and diversity index (Shannon index, P = 0.044) of urinary microbiome. Certain bacterial genera (e.g., Porphyromona and Prevotella) were significantly correlated with severity of OAB sub-symptoms. This study demonstrated that urinary microbiome was intimately correlated with the severity of OAB symptom and the increase of the diversity and richness of urinary microbiome was accompanied by more severe OAB symptoms, indicating that urinary dysbiosis may play pivotal roles in the deterioration of functional bladder diseases.
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影响因子：
2
作者：
An, Fang;Yang, Xin;Wang, Jian L.
通讯作者：
Wang, Jian L.
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL
影响因子：
7.2
作者：
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通讯作者：
Kenton, Kimberly
DOI：
10.1016/j.ejogrb.2017.04.040
发表时间：
2017-07-01
影响因子：
2.6
作者：
Curtiss, Natasha;Balachandran, Aswini;Duckett, Jonathan
通讯作者：
Duckett, Jonathan
膀胱过度活动症女性的尿液微生物组和心理因素
影响因子：
5.7
作者：
Wu P;Chen Y;Zhao J;Zhang G;Chen J;Wang J;Zhang H
通讯作者：
Zhang H