Urinary Microbiome and Psychological Factors in Women with Overactive Bladder.
Urinary Microbiome and Psychological Factors in Women with Overactive Bladder.
复制标题
膀胱过度活动症女性的尿液微生物组和心理因素
DOI：
10.3389/fcimb.2017.00488
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发表时间：
2017
影响因子：
5.7
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wu P;Chen Y;Zhao J;Zhang G;Chen J;Wang J;Zhang H
目的：新的证据表明，尿路微生物组的改变与下尿路症状有关。膀胱过度活动症(OAB)是一种常见疾病，病因复杂，常伴有心理疾病。需要更多关于OAB中的尿菌群和心理因素的信息。本研究的目的是描述女性OAB相关的尿菌群特征，并探讨尿菌群与心理因素的关系。方法：对30例OAB患者和25例无症状对照组进行膀胱过度活动症状评分、焦虑自评量表和抑郁自评量表的测评。尿液标本经尿管插管后用Illumina MiSeq进行16S rRNA基因测序。使用QIIME处理测序读数。Lefse显示对照组和OAB患者在细菌属上有显著差异。用皮尔逊相关系数分析尿菌群多样性与心理评分之间的关系。结果：细菌多样性(Simpson指数)和细菌丰富度(Chao1)均低于对照组(P均=0.038)。OAB和对照细菌群落有显著差异(基于加权UniFrac距离度量，R=0.064，P=0.037)。Lefse显示，与对照组相比，OAB组增加了7个属(如变形杆菌和气球菌属)，减少了13个属(如乳杆菌和普氏杆菌)。OAB组抑郁自评量表评分与尿菌群丰富度(CHO1，r=−0.458，P=0.011)和多样性(香农指数，r=−0.516，P=0.003)呈负相关。有焦虑或抑郁的OAB妇女与无焦虑或抑郁的OAB妇女的某些细菌属有显著差异。结论：尿菌群异常，多样性和丰富度降低，可能在OAB的发病机制和治疗中具有重要意义。女性OAB患者的心理状况与尿菌群特征相关。需要进一步的研究来了解中枢神经系统和尿路微生物群之间的联系。
Objectives: Emerging evidence indicates that alterations to the urinary microbiome are related to lower urinary tract symptoms. Overactive bladder (OAB) is a common disorder with complex etiologies and usually accompanied by psychological diseases. More information concerning the urinary microbiome and psychological factors in OAB is required. The aim of this study was to characterize the female urinary microbiome associated with OAB and investigate the relationships between urinary microbiome and psychological factors. Methods: Thirty women with OAB and 25 asymptomatic controls were recruited and asked to finish the Overactive Bladder Symptom Score, Self-Rating Anxiety Scale and Self-Rating Depression Scale. Urine specimens were collected by transurethral catheterization and processed for 16S rRNA gene sequencing using Illumina MiSeq. Sequencing reads were processed using QIIME. LEfSe revealed significant differences in bacterial genera between controls and OAB patients. The relationships between the diversity of the urinary microbiome and psychological scores were identified by Pearson's correlation coefficient. Results: We found that bacterial diversity (Simpson index) and richness (Chao1) were lower in OAB samples compared to controls (P both = 0.038). OAB and control bacterial communities were significantly different (based on weighted UniFrac distance metric, R = 0.064, P = 0.037). LEfSe demonstrated that 7 genera were increased (e.g., Proteus and Aerococcus) and 13 were reduced (e.g., Lactobacillus and Prevotella) in OAB group compared to controls. There were negative correlations between scores on Self-Rating Depression Scale and both richness (Chao1, r = −0.458, P = 0.011) and diversity (Shannon index, r = −0.516, P = 0.003) of urinary microbiome in OAB group. Some bacterial genera of OAB women with anxiety or depression were significantly different from those without. Conclusions: The aberrant urinary microbiome with decreased diversity and richness may have strong implications in pathogenesis and treatment of OAB. Psychological conditions were correlated with characteristics of urinary microbiome in women with OAB. Further research is needed to understand the connection between central nervous system and urinary microbiome.
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DOI：
10.1016/j.juro.2013.08.024
发表时间：
2014-02
期刊：
The Journal of urology
影响因子：
--
作者：
Ness TJ;Lloyd LK;Fillingim RB
通讯作者：
Fillingim RB
影响因子：
6.6
作者：
Nickel, J. Curtis;Stephens, Alisa;Landis, J. Richard;Chen, Jun;Mullins, Chris;van Bokhoven, Adrie;Lucia, M. Scott;Melton-Kreft, Rachael;Ehrlichk, Garth D.
通讯作者：
Ehrlichk, Garth D.
影响因子：
5.7
作者：
Lewis DA;Brown R;Williams J;White P;Jacobson SK;Marchesi JR;Drake MJ
通讯作者：
Drake MJ
影响因子：
6.6
作者：
Khasriya, Rajvinder;Khan, Shozab;Malone-Lee, James
通讯作者：
Malone-Lee, James
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL