Evaluation of the GenoType NTM-DR assay performance for the identification and molecular detection of antibiotic resistance in Mycobacterium abscessus complex.
Evaluation of the GenoType NTM-DR assay performance for the identification and molecular detection of antibiotic resistance in Mycobacterium abscessus complex.
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DOI：
10.1371/journal.pone.0239146
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Godreuil S
中科院分区：
文献类型：
--
作者：
Bouzinbi N;Marcy O;Bertolotti T;Chiron R;Bemer P;Pestel-Caron M;Peuchant O;Guet-Revillet H;Fangous MS;Héry-Arnaud G;Ouedraogo AS;Bañuls AL;Godreuil S
本研究的第一个目标是确定用于脓肿分枝杆菌复合分离株亚种鉴定的NTM-DR基因分型检测性能。第二个目的是通过药敏试验(DST)和ERM(41)、RRL和RRS基因的PCR测序，评价NTM-DR基因检测克拉霉素和阿米卡星耐药的能力。NTM-DR与MLST亚种鉴定结果的符合率为100%。对RRL和RRS基因的野生型和突变等位基因进行NTM-DR检测和PCR测序，结果符合率为100%(115/115)。同样，在克拉霉素和阿米卡星检测中，NTM-DR和DST基因型之间的符合率为100%。检测克拉霉素和阿米卡星耐药性的敏感度为100%。NTM-DR基因分型方法为亚种鉴定和耐药性检测提供了一种可靠的金标准方法(MLST和肉汤微量稀释法)的补充工具。
The first objective of this study was to determine the GenoType NTM-DR assay performance for subspecies identification in Mycobacterium abscessus complex isolates. The second objective was to evaluate the GenoType NTM-DR assay ability to detect clarithromycin and amikacin resistance in M. abscessus complex isolates compared with drug susceptibility testing (DST) and PCR sequencing of the erm(41), rrl and rrs genes. The concordance between the GenoType NTM-DR and MLST results concerning subspecies identification was 100%. The wild type and mutated alleles of the rrl and rrs genes were detected by the GenoType NTM-DR assay and PCR sequencing with 100% (115/115) agreement. Similarly, 100% concordance between GenoType NTM-DR and DST was observed for clarithromycin and amikacin testing. Sensitivity for the detection of clarithromycin and amikacin resistance was 100%. The GenoType NTM-DR assay provides a robust and complementary tool to the gold standard methods (MLST and broth microdilution) for subspecies identification and drug resistance detection.
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影响因子：
11.8
作者：
Lee, Meng-Rui;Sheng, Wang-Huei;Hsueh, Po-Ren
通讯作者：
Hsueh, Po-Ren
影响因子：
10
作者：
Floto RA;Olivier KN;Saiman L;Daley CL;Herrmann JL;Nick JA;Noone PG;Bilton D;Corris P;Gibson RL;Hempstead SE;Koetz K;Sabadosa KA;Sermet-Gaudelus I;Smyth AR;van Ingen J;Wallace RJ;Winthrop KL;Marshall BC;Haworth CS;US Cystic Fibrosis Foundation and European Cystic Fibrosis Society
通讯作者：
US Cystic Fibrosis Foundation and European Cystic Fibrosis Society
影响因子：
5.2
作者：
Maurer, Florian P.;Rueegger, Vera;Boettger, Erik C.
通讯作者：
Boettger, Erik C.
影响因子：
9.4
作者：
Macheras, Edouard;Roux, Anne-Laure;Heym, Beate
通讯作者：
Heym, Beate
影响因子：
9.4
作者：
Rueger, Kai;Hampel, Annegret;Bange, Franz-Christoph
通讯作者：
Bange, Franz-Christoph