FoxO3a Serves as a Biomarker of Oxidative Stress in Human Lens Epithelial Cells under Conditions of Hyperglycemia.
FoxO3a Serves as a Biomarker of Oxidative Stress in Human Lens Epithelial Cells under Conditions of Hyperglycemia.
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DOI：
10.1371/journal.pone.0067126
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Abraham EC
中科院分区：
文献类型：
--
作者：
Raju I;Kannan K;Abraham EC
叉头框'0'转录因子（FoxOs）与2型糖尿病和其他代谢性疾病的发病机制有关。在葡萄糖和胰岛素代谢中报告了FoxOs的异常活性。FoxO蛋白在眼组织中的表达被报道，然而它们在高血糖条件下的功能没有被检查。利用人透镜上皮细胞系研究FoxO蛋白的功能。采用免疫荧光、流式细胞术和Western blotting检测高血糖状态下FoxO蛋白的表达。在这项研究中，我们研究了FoxO3a在高血糖诱导的人透镜上皮细胞氧化应激中的作用。FoxO3a蛋白表达在高糖处理后以剂量和时间依赖性方式升高。透镜上皮细胞的抗氧化防御机制被削弱，证明从线粒体膜完整性的损失和降低MnSOD处理72小时后，高葡萄糖。总之，FoxO 3 a作为糖尿病条件下人透镜上皮细胞中氧化应激和细胞稳态的敏感指标。FoxO3a是糖尿病条件下对葡萄糖毒性的早期应激反应蛋白。
Forkhead box ‘O’ transcription factors (FoxOs) are implicated in the pathogenesis of type2 diabetes and other metabolic diseases. Abnormal activity of FoxOs was reported in the glucose and insulin metabolism. Expression of FoxO proteins was reported in ocular tissues; however their function under hyperglycemic conditions was not examined. Human lens epithelial cell line was used to study the function of FoxO proteins. Immunofluorescence, flow cytometry and Western blotting were employed to detect the FoxO proteins under the conditions of hyperglycemia. In this study we examined the role of FoxO3a in hyperglycemia-induced oxidative stress in human lens epithelial cells. FoxO3a protein expression was elevated in a dose- and time-dependent fashion after high glucose treatment. Anti-oxidant defense mechanisms of the lens epithelial cells were diminished as evidenced from loss of mitochondrial membrane integrity and lowered MnSOD after 72 h treatment with high glucose. Taken together, FoxO3a acts as a sensitive indicator of oxidative stress and cell homeostasis in human lens epithelial cells during diabetic conditions. FoxO3a is an early stress response protein to glucose toxicity in diabetic conditions.
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叉头转录因子在糖尿病诱导的氧化应激中的作用。
影响因子：
--
作者：
Ponugoti B;Dong G;Graves DT
通讯作者：
Graves DT
影响因子：
64.5
作者：
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通讯作者：
DePinho, Ronald A.
DOI：
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发表时间：
1995-07-06
影响因子：
3.1
作者：
CHERIAN, M;ABRAHAM, EC
通讯作者：
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影响因子：
56.9
作者：
Nemoto, S;Finkel, T
通讯作者：
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影响因子：
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作者：
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通讯作者：
Burgering, BMT