Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni.
Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni.
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DOI：
10.1128/spectrum.00203-22
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发表时间：
2022-06-29
影响因子：
3.7
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中科院分区：
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空肠弯曲菌在其整个传播和感染生命周期中所采用的生存策略在很大程度上仍然难以捉摸。具体地说，对于小非编码RNA(SRNAs)对应激适应的转录后调控缺乏了解。已发表的空肠弯曲菌sRNA已在特定条件下被发现，但对其生物学活动的了解有限。更多的sRNA尚未被发现，因为它们可能是依赖于条件的。在这里，我们已经从21种与宿主和传播相关的条件下生成了转录数据。这些数据揭示了不同胁迫条件下的转录起始位置、表达模式和转录后调控。这个数据集帮助预测了一系列可能的sRNA。通过整合差异基因表达分析、共表达分析和全基因组sRNA靶标预测，我们进一步探索了sRNAs的生物学功能。结果表明，营养剥夺和食物储存条件主要影响空肠弯曲菌基因的表达。进一步的探索揭示了一个可能的sRNA(CjSA21)，它在食品加工条件下针对tlp1到4。Tlp1到tlp4是编码甲基接受趋化蛋白(MCP)的转录本，MCP负责化学传感。这些结果表明，在食品加工条件下，CjSA21抑制趋化性并促进存活。这项研究为更广泛的研究社区提供了全面的数据集，并强调了一种新的sRNA作为潜在的趋化抑制物。重要性食源性致病菌空肠弯曲菌对全球卫生保健系统是一个重大挑战。为了了解空肠弯曲菌如何适应不同的胁迫条件，研究小RNA对其转录后调控是至关重要的。然而，可用于研究应激状态下sRNA活性的数据有限。在本研究中，我们建立了空肠弯曲菌在21种胁迫条件下的基因表达数据。我们的数据分析表明，其中一个新的sRNA介导了对食品加工条件的适应。我们的工作结果阐明了空肠弯曲菌的转录后调控，并确定了与食品安全相关的sRNA。
The survival strategies that Campylobacter jejuni (C. jejuni) employ throughout its transmission and infection life cycles remain largely elusive. Specifically, there is a lack of understanding about the posttranscriptional regulation of stress adaptations resulting from small noncoding RNAs (sRNAs). Published C. jejuni sRNAs have been discovered in specific conditions but with limited insights into their biological activities. Many more sRNAs are yet to be discovered as they may be condition-dependent. Here, we have generated transcriptomic data from 21 host- and transmission-relevant conditions. The data uncovered transcription start sites, expression patterns and posttranscriptional regulation during various stress conditions. This data set helped predict a list of putative sRNAs. We further explored the sRNAs’ biological functions by integrating differential gene expression analysis, coexpression analysis, and genome-wide sRNA target prediction. The results showed that the C. jejuni gene expression was influenced primarily by nutrient deprivation and food storage conditions. Further exploration revealed a putative sRNA (CjSA21) that targeted tlp1 to 4 under food processing conditions. tlp1 to 4 are transcripts that encode methyl-accepting chemotaxis proteins (MCPs), which are responsible for chemosensing. These results suggested CjSA21 inhibits chemotaxis and promotes survival under food processing conditions. This study presents the broader research community with a comprehensive data set and highlights a novel sRNA as a potential chemotaxis inhibitor. IMPORTANCE The foodborne pathogen C. jejuni is a significant challenge for the global health care system. It is crucial to investigate C. jejuni posttranscriptional regulation by small RNAs (sRNAs) in order to understand how it adapts to different stress conditions. However, limited data are available for investigating sRNA activity under stress. In this study, we generate gene expression data of C. jejuni under 21 stress conditions. Our data analysis indicates that one of the novel sRNAs mediates the adaptation to food processing conditions. Results from our work shed light on the posttranscriptional regulation of C. jejuni and identify an sRNA associated with food safety.
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