Photothermal-chemotherapy with doxorubicin-loaded hollow gold nanospheres: A platform for near-infrared light-trigged drug release.
Photothermal-chemotherapy with doxorubicin-loaded hollow gold nanospheres: A platform for near-infrared light-trigged drug release.
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DOI：
10.1016/j.jconrel.2011.10.028
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发表时间：
2012-03-10
期刊：
影响因子：
--
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
You J;Zhang R;Zhang G;Zhong M;Liu Y;Van Pelt CS;Liang D;Wei W;Sood AK;Li C
光热消融（PTA）是一种利用近红外（NIR）激光产生的热量破坏肿瘤细胞的新兴技术。然而，用PTA完全根除肿瘤细胞是困难的，因为治疗体积中的热分布不均匀。我们假设，使用单一多功能纳米结构介导同时光热细胞杀伤和药物释放（光热化疗）将PTA与化疗相结合，与单独化疗相比，将导致增强的抗肿瘤活性和降低的毒性。将多柔比星（DOX）负载到用聚乙二醇（PEG）包覆的中空金纳米球（HAuNS）中。使用双同位素标记技术评价了DOX和HAuNS在所得纳米构建体（具有不同DOX：PEG：HAuNS比率的DOX@ PEG-HAuNS）中的药代动力学和生物分布。使用人MDA-MB-231乳腺癌和A2780卵巢癌细胞在体外和体内研究了DOX：PEG：HAuNS重量比为1：3：1（NP 3）的DOX@PEG-HAuNS与NIR激光的组合的抗肿瘤活性。在体外，NP 3介导癌细胞的PTA和NIR激光处理后的DOX释放。在体内，NP 3显示出比游离DOX更慢的血液清除和更大的肿瘤蓄积。NP 3加NIR激光显示出比游离DOX、NP 3或脂质体DOX更大的抗肿瘤活性。此外，与游离DOX或脂质体DOX相比，NP 3显示出显著降低的全身毒性。NP 3加激光增强的抗肿瘤作用可以归因于从NP 3释放的DOX的细胞毒作用和HAuNS介导的光热效应。在正常组织中，从NP 3缓慢释放DOX有助于降低全身毒性。以单药纳米构建体NP 3为例的光热化疗是一种有前途的抗癌治疗方法。
Photothermal ablation (PTA) is an emerging technique that uses near-infrared (NIR) laser light-generated heat to destroy tumor cells. However, complete eradication of tumor cells with PTA is difficult because of uneven heat distribution in the treatment volume. We hypothesized that combining PTA with chemotherapy using a single multifunctional nanoconstruct that mediates simultaneous photothermal cell killing and drug release (photothermal-chemotherapy) would result in enhanced antitumor activity and reduced toxicity compared to chemotherapy alone. Doxorubicin (DOX) was loaded to hollow gold nanospheres (HAuNS) coated with polyethylene glycol (PEG). The pharmacokinetics and biodistribution of both DOX and HAuNS in the resulting nanoconstruct, DOX@PEG-HAuNS having different DOX:PEG:HAuNS ratios, were evaluated using dual isotope labeling techniques. The antitumor activity of DOX@PEG-HAuNS with DOX:PEG:HAuNS weight ratio of 1:3:1 (NP3) in combination with NIR laser was studied in vitro and in vivo using human MDA-MB-231 breast cancer and A2780 ovarian cancer cells. In vitro, NP3 mediated PTA of both cancer cells and DOX release upon NIR laser treatment. In vivo, NP3 showed slower clearance in blood and greater accumulation in tumors than free DOX. NP3-plus-NIR laser demonstrated greater antitumor activity than free DOX, NP3, or liposomal DOX. Moreover, NP3 displayed significantly decreased systemic toxicity compared to free DOX or liposomal DOX. Enhanced antitumor effect with NP3-plus-laser can be attributed to both the cytotoxic effect of DOX released from NP3 and the photothermal effect mediated by HAuNS. Slow release of DOX from NP3 in normal tissues contributed to reduced systemic toxicity. Photothermal-chemotherapy exemplified by a single-agent nanoconstruct NP3 is a promising approach to anticancer therapy.
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影响因子：
11.2
作者：
Lu W;Zhang G;Zhang R;Flores LG 2nd;Huang Q;Gelovani JG;Li C
通讯作者：
Li C
影响因子：
9
作者：
Curley, SA;Izzo, F;Cremona, F
通讯作者：
Cremona, F
影响因子：
12.6
作者：
Lee, Sangyeop;Chon, Hyangah;Oh, Chil Hwan
通讯作者：
Oh, Chil Hwan
影响因子：
14
作者：
Lu, Wei;Huang, Qian;Ku, Geng;Wen, Xiaoxia;Zhou, Min;Guzatov, Dmitry;Brecht, Peter;Su, Richard;Oraevsky, Alexander;Wang, Lihong V.;Li, Chun
通讯作者：
Li, Chun
影响因子：
3.8
作者：
He, XM;Wolkers, WF;Bischof, JC
通讯作者：
Bischof, JC