Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheres.
Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheres.
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DOI：
10.1016/j.biomaterials.2009.12.007
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发表时间：
2010-03
期刊：
影响因子：
14
通讯作者：
Li, Chun
中科院分区：
文献类型：
--
作者：
Lu, Wei;Huang, Qian;Ku, Geng;Wen, Xiaoxia;Zhou, Min;Guzatov, Dmitry;Brecht, Peter;Su, Richard;Oraevsky, Alexander;Wang, Lihong V.;Li, Chun
光声层析成像（PAT）也被称为光声层析成像（OAT）是一种混合成像模式，采用非电离光辐射和超声检测。在这里，我们描述了一类新的光学造影剂的基础上介观空心金纳米球（HAuNS）的PAT的应用。HAuNS直径约为40 nm，内部中空，由薄的金壁组成。它们显示出调谐到近红外（NIR）范围的强共振吸收，吸收峰在800 nm处，其光声效率显著大于血液。在与硫醇化聚（乙二醇）表面缀合后，聚乙二醇化HAuNS（PEG-HAuNS）的分布和消除半衰期分别为1.38±0.38和71.82±30.46 h。与基于裸鼠内在光学对比度的PAT图像相比，静脉内施用PEG-HAuNS后2小时内获得的PAT图像显示了更清晰和详细的脑血管。该图像描绘了使用PEG-HAuNS作为造影剂的直径小至约100 µm的脑血管。初步结果显示，在单次成像剂量的PEG-HAuNS后，对小鼠的肝脏、脾脏或肾脏没有急性毒性。我们的研究结果表明，PEG-HAuNS是有前途的对比剂PAT，具有高的空间分辨率和增强的灵敏度。
Photoacoustic tomography (PAT) also referred to as optoacoustic tomography (OAT) is a hybrid imaging modality that employs nonionizing optical radiation and ultrasonic detection. Here, we describe the application of a new class of optical contrast agents based on mesoscopic hollow gold nanospheres (HAuNS) to PAT. HAuNS are ~40 nm in diameter with a hollow interior and consist of a thin gold wall. They display strong resonance absorption tuned to the near infrared (NIR) range, with an absorption peak at 800 nm, whose photoacoustic efficiency is significantly greater than that of blood. Following surface conjugation with thiolated poly(ethylene glycol), the pegylated HAuNS (PEG-HAuNS) had distribution and elimination half-lives of 1.38±0.38 and 71.82±30.46 h, respectively. Compared with PAT images based on the intrinsic optical contrast in nude mice, the PAT images acquired within 2 h after intravenous administration of PEG-HAuNS showed the brain vasculature with greater clarity and detail. The image depicted brain blood vessels as small as ~100 µm in diameter using PEG-HAuNS as contrast agents. Preliminary results showed no acute toxicity to the liver, spleen, or kidneys in mice following a single imaging dose of PEG-HAuNS. Our results indicate that PEG-HAuNS are promising contrast agents for PAT, with high spatial resolution and enhanced sensitivity.
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影响因子：
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