Heterosubtypic antibody response elicited with seasonal influenza vaccine correlates partial protection against highly pathogenic H5N1 virus.
Heterosubtypic antibody response elicited with seasonal influenza vaccine correlates partial protection against highly pathogenic H5N1 virus.
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DOI：
10.1371/journal.pone.0017821
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发表时间：
2011-03-25
期刊：
影响因子：
3.7
通讯作者：
Zhou P
中科院分区：
文献类型：
--
作者：
Ding H;Tsai C;Zhou F;Buchy P;Deubel V;Zhou P
高致病性禽流感 (HPAI) H5N1 病毒在人类中的传播仍然是一个全球健康问题。季节性流感疫苗和潜在大流行性流感病毒之间的异亚型抗体反应对公共卫生具有重要影响。 Corti 等人之前的研究。和乔亚等人。证明人类季节性流感疫苗可以引发针对高致病性 H5N1 病毒的异亚型中和抗体。然而，这种反应是否能提供针对高致病性 H5N1 病毒的免疫保护仍有待确定。在这项研究中，我们使用基于流感 HA（血凝素）和 NA（神经氨酸酶）假型中和 (PN) 检测的敏感流感病毒，首次证实季节性流感疫苗确实在人类中引发了针对 H5N1 病毒的低水平异亚型中和抗体反应。然后，我们用季节性流感疫苗对小鼠进行免疫，并用致死剂量的高致病性 H5N1 病毒攻击它们。作为对照，我们用同亚型 H5N1 病毒样颗粒 (VLP) 或 PBS 免疫小鼠，并用相同的 H5N1 病毒攻击它们。在这里，我们发现季节性流感疫苗在小鼠体内引发了低水平的异亚型中和抗体反应，该反应明显高于 PBS 对照中的反应。其中，27 人中的 2 人的免疫血清表现出与 VLP 对照相似水平的中和抗体反应，实际上从高致病性 H5N1 病毒攻击中幸存下来。因此，我们得出结论，季节性流感疫苗确实在人类和小鼠中引发了低水平的异亚型中和抗体反应，并且在一定水平上，这种反应提供了针对 H5N1 病毒严重感染的免疫保护。
The spread of highly pathogenic avian influenza (HPAI) H5N1 virus in human remains a global health concern. Heterosubtypic antibody response between seasonal influenza vaccine and potential pandemic influenza virus has important implications for public health. Previous studies by Corti et al. and by Gioia et al. demonstrate that heterosubtypic neutralizing antibodies against the highly pathogenic H5N1 virus can be elicited with a seasonal influenza vaccine in humans. However, whether such response offers immune protection against highly pathogenic H5N1 virus remained to be determined. In this study, using a sensitive influenza HA (hemagglutinin) and NA (neuraminidase) pseudotype-based neutralization (PN) assay we first confirmed that low levels of heterosubtypic neutralizing antibody response against H5N1 virus were indeed elicited with seasonal influenza vaccine in humans. We then immunized mice with the seasonal influenza vaccine and challenged them with lethal doses of highly pathogenic H5N1 virus. As controls, we immunized mice with homosubtypic H5N1 virus like particles (VLP) or PBS and challenged them with the same H5N1 virus. Here we show that low levels of heterosubtypic neutralizing antibody response were elicited with seasonal influenza vaccine in mice, which were significantly higher than those in PBS control. Among them 2 out of 27 whose immune sera exhibited similar levels of neutralizing antibody response as VLP controls actually survived from highly pathogenic H5N1 virus challenge. Therefore, we conclude that low levels of heterosubtypic neutralizing antibody response are indeed elicited with seasonal influenza vaccine in humans and mice and at certain levels such response offers immune protection against severity of H5N1 virus infection.
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