Genome-wide identification and comprehensive analysis of NAC family genes involved in fruit development in kiwifruit (Actinidia).
Genome-wide identification and comprehensive analysis of NAC family genes involved in fruit development in kiwifruit (Actinidia).
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DOI：
10.1186/s12870-020-02798-2
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发表时间：
2021-01-15
影响因子：
5.3
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Jia D;Jiang Z;Fu H;Chen L;Liao G;He Y;Huang C;Xu X
NAC转录因子是由一个大的基因家族编码的植物特异性蛋白质。它们在植物的生长发育、叶片衰老以及对生物或非生物胁迫的响应等多种生物学过程中发挥着重要作用。已经主要在模式植物中鉴定了许多NAC TF的功能。然而，很少有研究NAC TF已在猕猴桃果树进行。鉴定了全基因组NAC基因，并分析了它们的同源性、基因组结构、染色体定位、同线性关系、蛋白质性质和保守基序。此外，还对毛花猕猴桃新品种‘甘露1号’果实发育过程进行了研究。并采用实时荧光定量PCR方法分析了这些NAC基因在甘露1号果实发育过程中的表达情况。我们的研究鉴定了142个NAC转录因子，它们在遗传学上可分为23个蛋白质亚家族。这些NAC基因的基因组结构表明它们的外显子在1到10之间。染色体定位分析表明，142个NAC中有116个分布在猕猴桃的29条染色体上。此外，全基因组基因表达分析表明，142个NAC基因中有125个在果实样品中表达。我们的综合研究提供了猕猴桃果实NAC基因和表达模式的新信息。本研究为进一步鉴定猕猴桃果实发育相关NAC基因的功能奠定了基础。
NAC transcription factors (TFs) are plant-specific proteins encoded by a large gene family. They play important roles in diverse biological processes, such as plant growth and development, leaf senescence, and responses to biotic or abiotic stresses. Functions of a number of NAC TFs have been identified mainly in model plants. However, very few studies on NAC TFs have been conducted in the fruit tree of kiwifruit. Genome-wide NAC genes were identified and their phylogeny, genomic structure, chromosomal location, synteny relationships, protein properties and conserved motifs were analyzed. In addition, the fruit developmental process was evaluated in a new kiwifruit cultivar of Actinidia eriantha ‘Ganlu 1’. And expressions for all those NAC genes were analyzed by quantitative real-time PCR method in fruits of ‘Ganlu 1’ during its developmental process. Our research identified 142 NAC TFs which could be phylogenetically divided into 23 protein subfamilies. The genomic structures of those NAC genes indicated that their exons were between one and ten. Analysis of chromosomal locations suggested that 116 out of 142 NACs distributed on all the 29 kiwifruit chromosomes. In addition, genome-wide gene expression analysis showed that expressions of 125 out of 142 NAC genes could be detected in fruit samples. Our comprehensive study provides novel information on NAC genes and expression patterns in kiwifruit fruit. This research would be helpful for future functional identification of NAC genes involved in kiwifruit fruit development.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
Horticultural Reviews
影响因子：
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作者：
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通讯作者：
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