Arabidopsis AtNAP regulates fruit senescence.
Arabidopsis AtNAP regulates fruit senescence.
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拟南芥 AtNAP 调控果实衰老
DOI：
10.1093/jxb/ers266
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发表时间：
2012-10
影响因子：
6.9
通讯作者：
Gan SS
中科院分区：
文献类型：
--
作者：
Kou X;Watkins CB;Gan SS
多不饱和脂肪酸（PUFA）是几种动物离子通道的强效调节剂。这里显示PUFA强烈影响由植物TPC 1基因编码的慢极性（SV）通道的活性。以花生四烯酸（AA）为PUFAs的模型分子，采用膜片钳技术对胡萝卜主根和拟南芥叶肉细胞的液泡进行了分离。我们的研究扩展到不同的PUFA，包括内源性α-亚麻酸（ALA）。微摩尔浓度的AA可逆地抑制SV通道，降低最大开放概率，使半激活电压变为正值。通过比较不同PUFA的作用，发现脂溶性酰基链的长度、双键的数目和极性头对通道的调节起关键作用，实验数据可以用一个简单的三态模型来重现，其中PUFA不直接与电压传感器相互作用，但影响通道从开放态到闭合态的电压无关性转变.结果表明，脂质在协调离子通道活动中起着重要作用，类似于从动物细胞中已知的。
Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana mesophyll cells and arachidonic acid (AA) was chosen as a model molecule for PUFAs. Our study was extended to different PUFAs including the endogenous alpha-linolenic acid (ALA). The addition of micromolar concentrations of AA reversibly inhibited the SV channel decreasing the maximum open probability and shifting the half activation voltage to positive values. Comparing the effects of different PUFAs, it was found that the length of the lipophilic acyl chain, the number of double bonds and the polar head were critical for channel modulation.The experimental data can be reproduced by a simple three-state model, in which PUFAs do not interact directly with the voltage sensors but affect the voltage-independent transition that leads the channel from the open state to the closed configuration. The results indicate that lipids play an important role in co-ordinating ion channel activities similar to what is known from animal cells.
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影响因子：
7.4
作者：
Fang, SC;Fernandez, DE
通讯作者：
Fernandez, DE
影响因子：
5.3
作者：
Carbonell-Bejerano P;Urbez C;Granell A;Carbonell J;Perez-Amador MA
通讯作者：
Perez-Amador MA
影响因子：
6.9
作者：
Jing, HC;Schippers, JHM;Dijkwei, PP
通讯作者：
Dijkwei, PP
影响因子：
7.2
作者：
PICTON, S;BARTON, SL;GRIERSON, D
通讯作者：
GRIERSON, D
影响因子：
3.1
作者：
Kumar, Rahul;Sharma, Manoj K.;Sharma, Arun K.
通讯作者：
Sharma, Arun K.