Effect of low-magnitude, high-frequency vibration on osteocytes in the regulation of osteoclasts.
Effect of low-magnitude, high-frequency vibration on osteocytes in the regulation of osteoclasts.
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DOI：
10.1016/j.bone.2010.02.031
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发表时间：
2010-06
期刊：
影响因子：
4.1
通讯作者：
You, Lidan
中科院分区：
文献类型：
--
作者：
Lau, Esther;Al-Dujaili, Saja;Guenther, Axel;Liu, Dawei;Wang, Liyun;You, Lidan
骨细胞被证明是骨中主要的力学传感器，负责向执行骨形成和吸收的效应细胞（成骨细胞和破骨细胞）发送信号。与这一假设相一致的是，已经表明骨细胞释放各种可溶性因子（例如转化生长因子-β、一氧化氮和胰高血糖素），当受到各种机械刺激（包括流体流动、静水压力和机械拉伸）时，这些因子影响成骨细胞和骨细胞的活性。最近，低幅高频（LMHF）振动（例如，加速度小于1g，其中g= 9.98m/s2，在20-90 Hz下）已经获得了很多关注，因为研究已经表明这种机械刺激可以积极地影响动物和人类的骨骼稳态。虽然LMHF振动的合成代谢和抗吸收潜力越来越明显，但介导骨适应LMHF振动的信号通路尚不清楚。我们假设骨细胞是机械传感器，负责检测振动刺激和产生可溶性因子，调节效应细胞的活性。因此，我们以各种频率（30、60、90 Hz）对骨细胞样MLO-Y 4细胞施加低幅度（0.3g）振动1小时。我们发现骨细胞在转录水平上对这种振动刺激敏感：考克斯-2在90 Hz时最大增加344%，而RANKL在60 Hz时最显著降低（-55%，p <0.01）。从振动的MLO-Y 4细胞收集的条件培养基使大破骨细胞（≥10个核）的形成减少36%（p<0.05），并且使骨吸收量减少20%（p=0.07）。发现振动组中条件培养基中可溶性RANKL（sRANKL）的量降低53%（p<0.01），而PGE 2释放也显著降低（-61%，p<0.01）。我们的结论是，骨细胞能够感知LMHF振动，并通过产生抑制破骨细胞形成的可溶性因子作出反应。
Osteocytes are well evidenced to be the major mechanosensor in bone, responsible for sending signals to the effector cells (osteoblasts and osteoclasts) that carry out bone formation and resorption. Consistent with this hypothesis, it has been shown that osteocytes release various soluble factors (e.g. transforming growth factor-β, nitric oxide, and prostaglandins) that influence osteoblastic and osteoclastic activities when subjected to a variety of mechanical stimuli, including fluid flow, hydrostatic pressure, and mechanical stretching. Recently, low-magnitude, high-frequency (LMHF) vibration (e.g., acceleration less than <1g, where g=9.98 m/s2, at 20-90 Hz) has gained much interest as studies have shown that such mechanical stimulation can positively influence skeletal homeostasis in animals and humans. Although the anabolic and anti-resorptive potential of LMHF vibration is becoming apparent, the signaling pathways that mediate bone adaptation to LMHF vibration are unknown. We hypothesize that osteocytes are the mechanosensor responsible for detecting the vibration stimulation and producing soluble factors that modulate the activity of effector cells. Hence, we applied low-magnitude (0.3g) vibrations to osteocyte-like MLO-Y4 cells at various frequencies (30, 60, 90 Hz) for 1 hour. We found that osteocytes were sensitive to this vibration stimulus at the transcriptional level: COX-2 maximally increased by 344% at 90 Hz, while RANKL decreased most significantly (-55%, p<0.01) at 60 Hz. Conditioned medium collected from the vibrated MLO-Y4 cells attenuated the formation of large osteoclasts (≥10 nuclei) by 36% (p<0.05) and the amount of osteoclastic resorption by 20% (p=0.07). The amount of soluble RANKL (sRANKL) in the conditioned medium was found to be 53% lower in the vibrated group (p<0.01), while PGE2 release was also significantly decreased (-61%, p<0.01). We conclude that osteocytes are able to sense LMHF vibration and respond by producing soluble factors that inhibit osteoclast formation.
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影响因子：
5.6
作者：
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影响因子：
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DOI：
10.1016/j.bbrc.2004.01.138
发表时间：
2004-03-19
影响因子：
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作者：
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通讯作者：
Klein-Nulend, J