Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor.
Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor.
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DOI：
10.3389/fpls.2018.01494
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发表时间：
2018
影响因子：
5.6
通讯作者：
Svistoonoff S
中科院分区：
文献类型：
--
作者：
Cissoko M;Hocher V;Gherbi H;Gully D;Carré-Mlouka A;Sane S;Pignoly S;Champion A;Ngom M;Pujic P;Fournier P;Gtari M;Swanson E;Pesce C;Tisa LS;Sy MO;Svistoonoff S
放线菌植物能够与Frankia细菌建立共生关系，导致根瘤的形成。共生相互作用始于植物和细菌之间在土壤中交换共生信号。这种分子对话涉及负责植物宿主及其内共生体的特异性识别的信号分子。本文研究了两个可能参与Frankia massarinae与其放线根宿主木麻黄glauca之间信号传递的因子：（1）根毛变形因子（CgRHDF）。青冈根毛接种F.和（2）NIN激活因子（CgNINA），其能够激活CgNIN的表达，CgNIN是在根毛发育的感染前阶段期间表达的共生基因。我们发现CgRHDF和CgNINA对应于小的耐热分子。这两个因素也是亲水性和耐几丁质酶消化的根瘤菌结瘤因子（NF）或菌根真菌LCO的结构差异。我们还调查了CgNINA和CgRHDF的存在下，在16个Frankia菌株的Frankia多样性的代表。对于来自进化枝Ic的木麻黄感染性菌株和来自进化枝Ia的密切相关菌株，检测到高水平的根毛变形（RHD）和ProCgNIN的活化，所述近缘菌株不能使C. glauca。属于进化枝III的远亲菌株水平较低。没有CgRHDF或CgNINA可以检测到的Frankia coriariae（进化枝II）或来自进化枝IV的非感染性菌株。
Actinorhizal plants are able to establish a symbiotic relationship with Frankia bacteria leading to the formation of root nodules. The symbiotic interaction starts with the exchange of symbiotic signals in the soil between the plant and the bacteria. This molecular dialog involves signaling molecules that are responsible for the specific recognition of the plant host and its endosymbiont. Here we studied two factors potentially involved in signaling between Frankia casuarinae and its actinorhizal host Casuarina glauca: (1) the Root Hair Deforming Factor (CgRHDF) detected using a test based on the characteristic deformation of C. glauca root hairs inoculated with F. casuarinae and (2) a NIN activating factor (CgNINA) which is able to activate the expression of CgNIN, a symbiotic gene expressed during preinfection stages of root hair development. We showed that CgRHDF and CgNINA corresponded to small thermoresistant molecules. Both factors were also hydrophilic and resistant to a chitinase digestion indicating structural differences from rhizobial Nod factors (NFs) or mycorrhizal Myc-LCOs. We also investigated the presence of CgNINA and CgRHDF in 16 Frankia strains representative of Frankia diversity. High levels of root hair deformation (RHD) and activation of ProCgNIN were detected for Casuarina-infective strains from clade Ic and closely related strains from clade Ia unable to nodulate C. glauca. Lower levels were present for distantly related strains belonging to clade III. No CgRHDF or CgNINA could be detected for Frankia coriariae (Clade II) or for uninfective strains from clade IV.
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影响因子：
5.2
作者：
Ktari A;Gueddou A;Nouioui I;Miotello G;Sarkar I;Ghodhbane-Gtari F;Sen A;Armengaud J;Gtari M
通讯作者：
Gtari M
影响因子：
3.5
作者：
Imanishi, Leandro;Vayssieres, Alice;Svistoonoff, Sergio
通讯作者：
Svistoonoff, Sergio
DOI：
10.1111/nph.13464
发表时间：
2015-08
期刊：
The New phytologist
影响因子：
--
作者：
Granqvist E;Sun J;Op den Camp R;Pujic P;Hill L;Normand P;Morris RJ;Downie JA;Geurts R;Oldroyd GE
通讯作者：
Oldroyd GE
影响因子：
3.5
作者：
Alloisio, Nicole;Queiroux, Clothilde;Kucho, Ken-ichi
通讯作者：
Kucho, Ken-ichi
影响因子：
56.9
作者：
Griesmann, Maximilian;Chang, Yue;Cheng, Shifeng
通讯作者：
Cheng, Shifeng