Metabolic Regulation of "Ca. Methylacidiphilum Fumariolicum" SolV Cells Grown Under Different Nitrogen and Oxygen Limitations.
Metabolic Regulation of "Ca. Methylacidiphilum Fumariolicum" SolV Cells Grown Under Different Nitrogen and Oxygen Limitations.
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DOI：
10.3389/fmicb.2012.00266
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发表时间：
2012
影响因子：
5.2
通讯作者：
Op den Camp HJ
中科院分区：
文献类型：
--
作者：
Khadem AF;Pol A;Wieczorek AS;Jetten MS;Op den Camp HJ
需氧甲烷氧化细菌可以使用甲烷作为其唯一的能源。 “Ca. Mmethylacidiphilum fumariolicum”菌株 SolV 和其他疣状微生物甲烷氧化菌的发现揭示了细菌氧化 CH4 的能力比之前在生态学、系统发育和生理学方面的假设更加多样化。甲烷氧化疣微菌的一个显着特征是其极端嗜酸表型，即使在 pH 1 以下也能生长。在本研究中，我们使用 RNA-Seq 分析了“Ca. M. fumariolicum”SolV 细胞的代谢调节，这些细胞在 pH 2 的分批培养中或在恒化器的固氮或限氧条件下以 μmax 生长。分析表明，编码颗粒甲烷单加氧酶的三个 pmoCAB 操纵子中的两个存在差异表达，可能由可用氧气调节。 N2 固定过程中产生的氢气显然被循环利用，编码 Ni/Fe 依赖性氢化酶的基因上调证明了这一点。这些氢化酶基因在低氧条件下也上调。亚硝化应激的处理通过在所有测试条件下一氧化氮还原酶编码基因norB和norC的表达、在氧限制下亚硝酸还原酶nirK的上调和在铵存在下羟胺氧化还原酶hao的上调来显示。解开碳氮代谢的基因调控有助于了解 SolV 菌株在自然生态系统恶劣条件下的潜在生理适应。
Aerobic methanotrophic bacteria can use methane as their sole energy source. The discovery of “Ca. Methylacidiphilum fumariolicum” strain SolV and other verrucomicrobial methanotrophs has revealed that the ability of bacteria to oxidize CH4 is much more diverse than has previously been assumed in terms of ecology, phylogeny, and physiology. A remarkable characteristic of the methane-oxidizing Verrucomicrobia is their extremely acidophilic phenotype, growing even below pH 1. In this study we used RNA-Seq to analyze the metabolic regulation of “Ca. M. fumariolicum” SolV cells growing at μmax in batch culture or under nitrogen fixing or oxygen limited conditions in chemostats, all at pH 2. The analysis showed that two of the three pmoCAB operons each encoding particulate methane monoxygenases were differentially expressed, probably regulated by the available oxygen. The hydrogen produced during N2 fixation is apparently recycled as demonstrated by the upregulation of the genes encoding a Ni/Fe-dependent hydrogenase. These hydrogenase genes were also upregulated under low oxygen conditions. Handling of nitrosative stress was shown by the expression of the nitric oxide reductase encoding genes norB and norC under all conditions tested, the upregulation of nitrite reductase nirK under oxygen limitation and of hydroxylamine oxidoreductase hao in the presence of ammonium. Unraveling the gene regulation of carbon and nitrogen metabolism helps to understand the underlying physiological adaptations of strain SolV in view of the harsh conditions of its natural ecosystem.
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影响因子：
5.5
作者：
Hou S;Makarova KS;Saw JH;Senin P;Ly BV;Zhou Z;Ren Y;Wang J;Galperin MY;Omelchenko MV;Wolf YI;Yutin N;Koonin EV;Stott MB;Mountain BW;Crowe MA;Smirnova AV;Dunfield PF;Feng L;Wang L;Alam M
通讯作者：
Alam M
DOI：
10.1099/mic.0.050278-0
发表时间：
2011-10
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Chaudhuri RR;Yu L;Kanji A;Perkins TT;Gardner PP;Choudhary J;Maskell DJ;Grant AJ
通讯作者：
Grant AJ
影响因子：
2.8
作者：
Hanczár, T;Csáki, R;Kovács, KL
通讯作者：
Kovács, KL
影响因子：
3.3
作者：
Belova, Svetlana E.;Baani, Mohamed;Dedysh, Svetlana N.
通讯作者：
Dedysh, Svetlana N.
影响因子：
56.9
作者：
Dekas, Anne E.;Poretsky, Rachel S.;Orphan, Victoria J.
通讯作者：
Orphan, Victoria J.