Retrovirus activation in embryonal carcinoma cells by cellular promoters.
Retrovirus activation in embryonal carcinoma cells by cellular promoters.
复制标题
细胞启动子在胚胎癌细胞中激活逆转录病毒。
作者：
Ivan Peckham;Suzanne Sobel;Jeff Comer;Rudolf Jaenisch;Eric Barklis
Retrovirus expression in embryonal carcinoma (EC) cells is blocked at a postintegration stage of the viral life cycle, because of the inadequate function of the viral long terminal repeat (LTR) promoter in this cell type. However, rare sites in the EC cell genome permit provirus expression by undefined mechanisms. Our analysis of three expressed proviruses indicates that they have inserted into actively transcribed regions. Two of the three, examined in detail, integrated into the first introns of cellular transcription units in close proximity to active cellular promoters. One of these cellular genes is the probable murine homolog of the yeast ribosomal protein L3, responsible for trichodermin resistance. In all cases, virus activation appears to involve production of viral transcripts that are initiated in the 5'-flanking region, transcribed through the viral LTR, and subsequently spliced from a cellular donor to a viral acceptor. Our results suggest a general procedure for the isolation of active genes and promoters in different tissues.
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影响因子：
56.9
作者：
M. Lenardo;L. Staudt;P. Robbins;A. Kuang;R. Mulligan;D. Baltimore
通讯作者：
M. Lenardo;L. Staudt;P. Robbins;A. Kuang;R. Mulligan;D. Baltimore
DOI：
10.1073/pnas.78.12.7634
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S
DOI：
10.1089/dna.1988.7.261
发表时间：
1988-05-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
DANIELSON, PE;FORSSPETTER, S;SUTCLIFFE, JG
通讯作者：
SUTCLIFFE, JG