Evidence for a dispersed Hox gene cluster in the platyhelminth parasite Schistosoma mansoni.
Evidence for a dispersed Hox gene cluster in the platyhelminth parasite Schistosoma mansoni.
复制标题
扁形动物寄生虫曼氏血吸虫中存在分散的 Hox 基因簇的证据。
DOI：
10.1093/molbev/msi239
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发表时间：
2005
影响因子：
10.7
通讯作者：
G. Balavoine
中科院分区：
文献类型：
--
作者：
R. Pierce;Wenjie Wu;H. Hirai;A. Ivens;L. Murphy;C. Noël;D. Johnston;F. Artiguenave;M. Adams;J. Cornette;E. Viscogliosi;M. Capron;G. Balavoine
在迄今为止研究的大多数双侧性生物中，Hox基因被组织成基因组簇，并决定沿前后轴沿着的发育。有人认为，这种聚类，连同基因表达的空间和时间共线性，代表了祖先的条件。然而，在生物体的胚胎发生和谱系依赖的机制，用于确定细胞命运的衍生模式，时间共线性的表达可能会丢失和Hox集群组织中断，是蜕皮动物果蝇和秀丽隐杆线虫和尾索动物玻璃海鞘和Oikopleura dioica的情况。我们试图确定是否lophotrochozoan，扁平蠕虫寄生虫血吸虫曼氏血吸虫，拥有一个保守的或中断的Hox集群。利用聚合酶链反应（PCR）为基础的策略，我们已经克隆和表征了三个新的S。mansoni基因编码的直向同源果蝇唇（SmHox 1），变形（SmHox 4），和腹部A（SmHox 8），以及全长编码序列的Smox 1，我们确定为直向同源fushi tarazu。定量逆转录-PCR显示，这四个基因在所有生命周期阶段的表达，但表达水平的差异调节。系统发育分析和保守的“parapeptide”序列的C-末端的同源结构域SmHox 8和Smox 1支持的分组扁形动物内的lophotrochozoan分支。然而，细菌人工染色体（BAC）文库筛选后的基因组步行未能重建一个集群。对含有Hox基因的BAC克隆进行测序，在任何情况下，在同一克隆上都没有发现其他Hox基因。此外，SmHox 4和SmHox 8基因含有单个非常大的内含子（>40 kbp），进一步表明染色体Hox簇高度延伸。使用荧光原位杂交的Hox基因的染色体定位表明，SmHox 4和SmHox 8是在4号染色体的长臂上，而SmHox 1和Smox 1是在3号染色体上。可用基因组序列的计算机筛选证实了Southern印迹和BAC文库筛选的结果，表明没有SmHox 1，SmHox 4或SmHox 8的旁系同源物。因此，染色体Hox簇不会复制，而是在基因组中分散和解体。
In most bilaterian organisms so far studied, Hox genes are organized in genomic clusters and determine development along the anteroposterior axis. It has been suggested that this clustering, together with spatial and temporal colinearity of gene expression, represents the ancestral condition. However, in organisms with derived modes of embryogenesis and lineage-dependent mechanisms for the determination of cell fate, temporal colinearity of expression can be lost and Hox cluster organization disrupted, as is the case for the ecdysozoans Drosophila melanogaster and Caenorhabditis elegans and the urochordates Ciona intestinalis and Oikopleura dioica. We sought to determine whether a lophotrochozoan, the platyhelminth parasite Schistosoma mansoni, possesses a conserved or disrupted Hox cluster. Using a polymerase chain reaction (PCR)-based strategy, we have cloned and characterized three novel S. mansoni genes encoding orthologues of Drosophila labial (SmHox1), deformed (SmHox4), and abdominal A (SmHox8), as well as the full-length coding sequence of the previously described Smox1, which we identify as an orthologue of fushi tarazu. Quantitative reverse transcriptase-PCR showed that the four genes were expressed at all life-cycle stages but that levels of expression were differentially regulated. Phylogenetic analysis and the conservation of "parapeptide" sequences C-terminal to the homeodomains of SmHox8 and Smox1 support the grouping of platyhelminths within the lophotrochozoan clade. However, Bacterial Artificial Chromosome (BAC) library screening followed by genome walking failed to reconstitute a cluster. The BAC clones containing Hox genes were sequenced, and in no case were other Hox genes found on the same clone. Moreover, the SmHox4 and SmHox8 genes contained single very large introns (>40 kbp) further indicating that the schistosome Hox cluster is highly extended. Localization of the Hox genes to chromosomes using fluorescence in situ hybridization showed that SmHox4 and SmHox8 are on the long arm of chromosome 4, whereas SmHox1 and Smox1 are on chromosome 3. In silico screening of the available genome sequences corroborated results of Southern blotting and BAC library screening that indicate that there are no paralogues of SmHox1, SmHox4, or SmHox8. The schistosome Hox cluster is therefore not duplicated, but is both dispersed and disintegrated in the genome.
从母孢子囊中生产曼氏血吸虫子孢子囊，该母孢子囊与光滑双脐胚胎 (Bge) 细胞保持共生培养。
DOI：
--
发表时间：
1995
期刊：
The Journal of parasitology.
影响因子：
--
作者：
Yoshino,TP;Laursen,JR
通讯作者：
Laursen,JR
秀丽隐杆线虫胚胎轴向模式需要两个最近发现的后群 Hox 基因。
DOI：
10.1073/pnas.97.9.4499
发表时间：
2000
影响因子：
11.1
作者：
VanAuken,K;Weaver,DC;Edgar,LG;Wood,WB
通讯作者：
Wood,WB
水蛭分割：细胞谱系和复杂身体模式的形成。
影响因子：
2.7
作者：
Shankland,M
通讯作者：
Shankland,M