Variability in the Occupancy of Escherichia coli O157 Integration Sites by Shiga Toxin-Encoding Prophages.
Variability in the Occupancy of Escherichia coli O157 Integration Sites by Shiga Toxin-Encoding Prophages.
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DOI：
10.3390/toxins13070433
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发表时间：
2021-06-22
期刊：
影响因子：
4.2
通讯作者：
Manning SD
中科院分区：
文献类型：
--
作者：
Henderson ST;Singh P;Knupp D;Lacher DW;Abu-Ali GS;Rudrik JT;Manning SD
大肠杆菌O 157：H7菌株通常产生滋贺毒素，该毒素由作为原噬菌体插入多个位点的类杆状噬菌体上的基因编码。O 157菌株分为不同的分支，毒力不同。在此，我们使用PCR测定来检查代表9个进化枝的346个O 157菌株中的yehV、argW、wrbA和sbcB中的滋贺毒素（Stx）原噬菌体占有率。总体而言，yehV在大多数菌株中被占据（n = 334，96.5%），其次是wrbA（n = 213，61.6%）、argW（n = 103，29.8%）和sbcB（n = 93，26.9%）。十二占用配置文件中确定的频率不同，不同的进化枝。与其他进化枝相比，属于进化枝8的菌株更可能占据sbcB和argW位点（p < 0.0001），而进化枝2菌株更可能占据wrbA位点（p < 0.0001）。进化枝8菌株还具有多于基于stx变体的存在的预期数量的占据位点（p < 0.0001）。在进化枝8菌株中缺失占据yehV的20 kb非Stx前噬菌体导致stx 2表达降低约18倍。这些数据突出了Stx原噬菌体整合的复杂性，并证明了以前与溶血性尿毒症综合征相关的进化枝8菌株具有独特的Stx原噬菌体占用谱，可以影响stx 2表达。
Escherichia coli O157:H7 strains often produce Shiga toxins encoded by genes on lambdoid bacteriophages that insert into multiple loci as prophages. O157 strains were classified into distinct clades that vary in virulence. Herein, we used PCR assays to examine Shiga toxin (Stx) prophage occupancy in yehV, argW, wrbA, and sbcB among 346 O157 strains representing nine clades. Overall, yehV was occupied in most strains (n = 334, 96.5%), followed by wrbA (n = 213, 61.6%), argW (n = 103, 29.8%), and sbcB (n = 93, 26.9%). Twelve occupancy profiles were identified that varied in frequency and differed across clades. Strains belonging to clade 8 were more likely to have occupied sbcB and argW sites compared to other clades (p < 0.0001), while clade 2 strains were more likely to have occupied wrbA sites (p < 0.0001). Clade 8 strains also had more than the expected number of occupied sites based on the presence of stx variants (p < 0.0001). Deletion of a 20 kb non-Stx prophage occupying yehV in a clade 8 strain resulted in an ~18-fold decrease in stx2 expression. These data highlight the complexity of Stx prophage integration and demonstrate that clade 8 strains, which were previously linked to hemolytic uremic syndrome, have unique Stx prophage occupancy profiles that can impact stx2 expression.
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影响因子：
11.8
作者：
Ogura Y;Seto K;Morimoto Y;Nakamura K;Sato MP;Gotoh Y;Itoh T;Toyoda A;Ohnishi M;Hayashi T
通讯作者：
Hayashi T
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11.8
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影响因子：
10.7
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通讯作者：
Clawson ML