Control of ribosomal RNA synthesis by hematopoietic transcription factors.
Control of ribosomal RNA synthesis by hematopoietic transcription factors.
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DOI：
10.1016/j.molcel.2022.08.027
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发表时间：
2022-10-20
期刊：
影响因子：
16
通讯作者：
Paralkar, Vikram R.
中科院分区：
文献类型：
--
作者：
Antony, Charles;George, Subin S.;Blum, Justin;Somers, Patrick;Thorsheim, Chelsea L.;Wu-Corts, Dexter J.;Ali, Yuxi;Gao, Long;Lv, Kaosheng;Tremblay, Michel G.;Moss, Tom;Tan, Kai;Wilusz, Jeremy E.;Ganley, Austen R. D.;Pimkin, Maxim;Paralkar, Vikram R.
Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA Polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2200 ChIP-Seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA, and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of CEBPA (C/EBP alpha), a critical hematopoietic TF with conserved rDNA binding, caused rapid reduction in rRNA transcription due to reduced Pol I occupancy. Our work identifies numerous potential rRNA regulators, and provides a template for dissection of TF roles in rRNA transcription. Antony et al. mapped existing ChIP-seq datasets to human and mouse rDNA, and identified binding profiles of cell-type specific transcription factors (TFs). The myeloid TF CEBPA bound rDNA at a conserved motif, and its degradation reduced rDNA occupancy of the Pol I-RRN3 complex, and nascent 47S rRNA transcription.
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影响因子：
3.7
作者：
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通讯作者：
Kimura K
影响因子：
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GRUMMT, F
DOI：
10.1261/rna.067843.118
发表时间：
2018-12
期刊：
RNA (New York, N.Y.)
影响因子：
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作者：
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通讯作者：
Cohen-Tannoudji M
影响因子：
4.4
作者：
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通讯作者：
SYLVESTER, JE