Ultradian glucocorticoid exposure directs gene-dependent and tissue-specific mRNA expression patterns in vivo.
Ultradian glucocorticoid exposure directs gene-dependent and tissue-specific mRNA expression patterns in vivo.
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DOI：
10.1016/j.mce.2016.10.019
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发表时间：
2017-01-05
影响因子：
4.1
通讯作者：
Conway-Campbell, Becky L.
中科院分区：
文献类型：
--
作者：
George, Charlotte L.;Birnie, Matthew T.;Flynn, Benjamin P.;Kershaw, Yvonne M.;Lightman, Stafford L.;Conway-Campbell, Becky L.
在本文中，我们报告的差分解码的超日皮质酮信号的糖皮质激素靶组织。肾上腺切除大鼠的脉动皮质酮替代导致不同的动力学的Sgk1 mRNA的生产，与一个独特的脉动mRNA的诱导在垂体中观察到的非脉动诱导前额叶皮层（PFC）的配置文件。我们进一步报告的第一个证据，在体内的糖皮质激素靶基因的脉动转录抑制，与脉动调节Pomc转录垂体。我们已经探索了一个潜在的机制，在PFC和垂体之间的相同的转录本（Sgk1）的诱导动力学的差异。垂体和前额叶皮质中的糖皮质激素受体（GR）激活特征显著不同，在垂体中检测到的GR活性的动态范围显著更大，持续时间更短，与观察到的更明显的基因脉冲效应一致。在前额叶皮层中，Gilz mRNA的表达也是非脉动的，并且与Sgk1相比，其增加和减少的时间过程显著延迟，另外突出了超日糖皮质激素治疗期间的基因特异性调节动力学。糖皮质激素超日节律转录解码的体内研究。皮质酮脉冲引发基因和组织特异性mRNA表达模式。皮质酮脉冲诱导大鼠垂体中Pomc hnRNA的脉冲抑制。与PFC不同，垂体表现出脉动的Sgk1 mRNA表达水平。差异GR动力学是组织特异性转录反应的基础。
In this paper we report differential decoding of the ultradian corticosterone signal by glucocorticoid target tissues. Pulsatile corticosterone replacement in adrenalectomised rats resulted in different dynamics of Sgk1 mRNA production, with a distinct pulsatile mRNA induction profile observed in the pituitary in contrast to a non-pulsatile induction in the prefrontal cortex (PFC). We further report the first evidence for pulsatile transcriptional repression of a glucocorticoid-target gene in vivo, with pulsatile regulation of Pomc transcription in pituitary. We have explored a potential mechanism for differences in the induction dynamics of the same transcript (Sgk1) between the PFC and pituitary. Glucocorticoid receptor (GR) activation profiles were strikingly different in pituitary and prefrontal cortex, with a significantly greater dynamic range and shorter duration of GR activity detected in the pituitary, consistent with the more pronounced gene pulsing effect observed. In the prefrontal cortex, expression of Gilz mRNA was also non-pulsatile and exhibited a significantly delayed timecourse of increase and decrease when compared to Sgk1, additionally highlighting gene-specific regulatory dynamics during ultradian glucocorticoid treatment. In vivo study of transcriptional decoding of the glucocorticoid ultradian rhythm. Corticosterone pulses elicit gene and tissue-specific mRNA expression patterns. Corticosterone pulses induce pulsatile repression of Pomc hnRNA in rat pituitary. Unlike the PFC, the pituitary exhibits pulsatile Sgk1 mRNA expression levels. Differential GR dynamics underlie tissue-specific transcriptional responsiveness.
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影响因子：
3.7
作者：
McMaster, Andrew;Jangani, Maryam;Ray, David W.
通讯作者：
Ray, David W.
影响因子：
15.9
作者：
Ayroldi, Emira;Zollo, Ornella;Riccardi, Carlo
通讯作者：
Riccardi, Carlo
DOI：
10.1073/pnas.80.22.6982
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BIRNBERG, NC;LISSITZKY, JC;HERBERT, E
通讯作者：
HERBERT, E
影响因子：
16.2
作者：
McEwen BS;Morrison JH
通讯作者：
Morrison JH
影响因子：
3.2
作者：
Conway-Campbell BL;Sarabdjitsingh RA;McKenna MA;Pooley JR;Kershaw YM;Meijer OC;de Kloet ER;Lightman SL
通讯作者：
Lightman SL