Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids.
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids.
复制标题
DOI：
10.3390/pharmaceutics15010157
复制
发表时间：
2023-01-03
期刊：
影响因子：
5.4
通讯作者：
Archer M
中科院分区：
文献类型：
--
作者：
Alves MMS;Melo MN;Mertens HDT;Pereiro AB;Archer M
蛋白质天生不稳定，这限制了它们作为治疗剂的使用。然而，生物相容助溶剂或表面活性剂的使用可以通过稳定分子内和溶剂介导的相互作用来帮助规避这一问题。离子液体(ILS)被认为是共溶剂或表面活性化合物。在蛋白质存在的情况下，ILS可以对其复性、保质期、稳定性和酶活性产生有益的影响。在本文描述的工作中，我们使用小角X射线散射(SAXS)来监测不同浓度的IL与蛋白质模型、溶菌酶(Lys)和牛血清白蛋白(BSA)的聚集，并使用荧光显微镜来评估氟化IL(FILs)与Lys的胶束形成。此外，粗粒分子动力学(CG-MD)模拟提供了对Lys-FIL相互作用的更好理解。结果表明，在FILs存在的情况下，蛋白质保持了其球状结构，在较高的FILs浓度下，Lys有部分展开的迹象，BSA有致密化的迹象，弹性增加。赖氨酸被FIL包裹，从而增强了ILS在蛋白质药物配方中的潜力。
Proteins are inherently unstable, which limits their use as therapeutic agents. However, the use of biocompatible cosolvents or surfactants can help to circumvent this problem through the stabilization of intramolecular and solvent-mediated interactions. Ionic liquids (ILs) have been known to act as cosolvents or surface-active compounds. In the presence of proteins, ILs can have a beneficial effect on their refolding, shelf life, stability, and enzymatic activities. In the work described herein, we used small-angle X-ray scattering (SAXS) to monitor the aggregation of different concentrations of ILs with protein models, lysozyme (Lys) and bovine serum albumin (BSA), and fluorescence microscopy to assess micelle formation of fluorinated ILs (FILs) with Lys. Furthermore, coarse-grained molecular dynamics (CG-MD) simulations provided a better understanding of Lys–FIL interactions. The results showed that the proteins maintain their globular structures in the presence of FILs, with signs of partial unfolding for Lys and compaction for BSA with increased flexibility at higher FIL concentrations. Lys was encapsulated by FIL, thus reinforcing the potential of ILs to be used in the formulation of protein-based pharmaceuticals.
登录
查看更多内容
影响因子：
64.8
作者：
Harris CR;Millman KJ;van der Walt SJ;Gommers R;Virtanen P;Cournapeau D;Wieser E;Taylor J;Berg S;Smith NJ;Kern R;Picus M;Hoyer S;van Kerkwijk MH;Brett M;Haldane A;Del Río JF;Wiebe M;Peterson P;Gérard-Marchant P;Sheppard K;Reddy T;Weckesser W;Abbasi H;Gohlke C;Oliphant TE
通讯作者：
Oliphant TE
影响因子：
6.1
作者：
Konarev, PV;Volkov, VV;Svergun, DI
通讯作者：
Svergun, DI
影响因子：
8.4
作者：
Sindhu, Anamika;Kumar, Sumit;Venkatesu, Pannuru
通讯作者：
Venkatesu, Pannuru
DOI：
10.3390/molecules27030984
发表时间：
2022-02-01
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Han Q;Broomhall HC;Vieira Veríssimo N;Ryan TM;Drummond CJ;Pereira JFB;Greaves TL
通讯作者：
Greaves TL
影响因子：
5.9
作者：
Bui-Le, Liem;Clarke, Coby J.;Brohl, Andreas;Brogan, Alex P. S.;Arpino, James A. J.;Polizzi, Karen M.;Hallett, Jason P.
通讯作者：
Hallett, Jason P.