A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.
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DOI：
10.1371/journal.pone.0021072
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lu Z
中科院分区：
文献类型：
--
作者：
Guo L;Yang Q;Lu J;Li H;Ge Q;Gu W;Bai Y;Lu Z
为了深入了解异构体微小RNA（isomiR）谱以及具有3′末端添加的isomiR在子痫前期中的潜在作用，我们利用SOLiD测序平台对不同程度子痫前期的胎盘样本中的微小RNA（miRNA）库以及3′末端添加事件进行了全面的研究。
超过30%的isomiR被检测到具有3′非模板添加核苷酸，尤其是添加腺苷核苷酸。然而，这些经过修饰的isomiR在总miRNA表达中所占比例较低（<15%）。一般来说，从一个给定的miRNA位点可鉴定出1 - 3种丰富的isomiR，但其中没有一种被检测到具有3′末端添加。不同的miRNA显示出不同的isomiR谱和表达模式。最丰富的isomiR谱、isomiR图谱以及表达模式通常是稳定的，但在此我们发现样本之间存在一些例外情况，特别是在正常样本和患病样本之间。在isomiR水平上，我们在正常样本和患病样本之间以及轻度和重度样本之间检测到了一组不同的差异表达的修饰isomiR。对其实验验证的靶基因进行的基因本体论分析显示，特定生物过程类别富集。
多种isomiR的现象，尤其是具有3′末端添加的isomiR，并非是前体miRNA加工过程中的随机事件。多种isomiR和表达模式揭示了潜在的功能影响，应予以考虑。这项研究丰富了miRNA与人类疾病的关联，包括各种miRNA变体以及3′末端添加事件的潜在作用。
To gain insight into potential roles of isomiR spectrum and isomiRs with 3′ additions in pre-eclampsia, we performed a comprehensive survey of miRNA repertoire and 3′ addition events from placental samples with different degrees of pre-eclampsia by applying SOLiD sequencing platform. Over 30% isomiRs were detected with 3′ non-template additional nucleotides, especially for additional nucleotide of adenosine. However, these modified isomiRs showed a lower percentage of total miRNA expression (<15%). Generally, 1-3 abundant isomiRs from a given miRNA locus were identified, but none of them was detected with 3′ additions. Different miRNAs indicated various isomiR spectrums and expression patterns. The most abundant isomiR spectrum, isomiR profile and expression pattern always were stability, but herein we found several exceptions across samples, especially between normal and diseased samples. At isomiR level, we detected a distinct subset of differentially expressed modified isomiRs between normal and diseased samples or between mild and severe samples. Gene Ontology analysis of their experimentally validated target genes revealed enrichment for specific biological process categories. The phenomenon of multiple isomiRs, especially for isomiRs with 3′ additions, is not a random event during pre-miRNA processing. Varieties of isomiRs and expression patterns reveal potential functional implication and should be taken into account. The study enriches association of miRNAs and human disease, including potential roles of various miRNA variants and 3′ addition events.
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