Seed DNA damage responses promote germination and growth in Arabidopsis thaliana.
Seed DNA damage responses promote germination and growth in Arabidopsis thaliana.
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DOI：
10.1073/pnas.2202172119
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发表时间：
2022-07-26
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
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成功的发芽是作物生产和自然生态系统的基础。然而，耐脱水种子在与种子老化相关的静止期积累了惊人水平的基因组损伤。在这里，我们表明，种子表现出内在的基因组压力，这是失去了种子提前发芽。种子最大限度地减少分生组织破坏和延迟程序性细胞死亡，以促进根生长种子老化后早期发芽。这揭示了种子在生长和转录谱方面对DNA损伤的不同反应，这支持在植物生命周期的这个关键阶段快速建立幼苗。这些发现推进了我们对植物DNA损伤反应和种子寿命的理解，这对作物产量和植物在气候变化下的生存至关重要。种子干燥、静止的状态使植物胚能长期存活。然而，在静止期积累的惊人水平的基因组损伤损害发芽和威胁植物的生存。种子减轻这种损害的机制尚不清楚。在这里，我们发现，吸胀的拟南芥种子表现出高抗DNA损伤，这是失去了种子提前发芽，与细胞周期活性增加。与幼苗相比，我们表明种子通过减少分生组织破坏和延迟SOG 1依赖性程序性细胞死亡来最大限度地减少DNA损伤的影响。这促进了根的生长早postgermination。为了应对老化种子中自然积累的DNA损伤，SOG 1在萌发后激活细胞死亡。SOG 1活性对于促进成功的幼苗建立也是重要的。种子和幼苗的这些不同的细胞反应通过不同的DNA损伤转录谱来反映。DNA修复突变体的比较分析确定了主要基因组维护途径在萌发中的作用，但细胞毒性染色体断裂的修复对种子寿命是最重要的。总的来说，这些结果表明，种子中发生的高水平DNA损伤被低细胞周期活性、细胞周期检查点和DNA修复所抵消，从而促进成功的幼苗建立。我们的研究结果揭示了植物DNA损伤反应的生理意义和维持种子寿命的机制，这对植物种群在自然环境中的生存和气候变化下的可持续作物生产至关重要。
Successful germination underpins crop production and natural ecosystems. However, the desiccation-tolerant seed accumulates striking levels of genome damage in quiescence associated with seed aging. Here, we show that seeds display intrinsic resistance to genome stress, which is lost as seeds advance to germination. Seeds minimize meristem disruption and delay programmed cell death in response to seed aging to promote root growth early postgermination. This reveals distinct responses of seeds to DNA damage in terms of growth and transcriptional profiles, which support rapid seedling establishment at this crucial stage of the plant lifecycle. These findings advance our understanding of both plant DNA damage responses and seed longevity, important for crop yields and plant survival under changing climates. The desiccated, quiescent state of seeds confers extended survival of the embryonic plant. However, accumulation of striking levels of genome damage in quiescence impairs germination and threatens plant survival. The mechanisms by which seeds mitigate this damage remain unclear. Here, we reveal that imbibed Arabidopsis seeds display high resistance to DNA damage, which is lost as seeds advance to germination, coincident with increasing cell cycle activity. In contrast to seedlings, we show that seeds minimize the impact of DNA damage by reducing meristem disruption and delaying SOG1-dependent programmed cell death. This promotes root growth early postgermination. In response to naturally accumulated DNA damage in aging seeds, SOG1 activates cell death postgermination. SOG1 activities are also important for promoting successful seedling establishment. These distinct cellular responses of seeds and seedlings are reflected by different DNA damage transcriptional profiles. Comparative analysis of DNA repair mutants identifies roles of the major genome maintenance pathways in germination but that the repair of cytotoxic chromosomal breaks is the most important for seed longevity. Collectively, these results indicate that high levels of DNA damage incurred in seeds are countered by low cell cycle activity, cell cycle checkpoints, and DNA repair, promoting successful seedling establishment. Our findings reveal insight into both the physiological significance of plant DNA damage responses and the mechanisms which maintain seed longevity, important for survival of plant populations in the natural environment and sustainable crop production under changing climates.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
DOI：
10.1073/pnas.0909218106
发表时间：
2009-12-08
影响因子：
11.1
作者：
Fulcher, Nick;Sablowski, Robert
通讯作者：
Sablowski, Robert
影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
通讯作者：
Doerner, P
影响因子：
7.2
作者：
Cordoba-Canero, Dolores;Roldan-Arjona, Teresa;Ariza, Rafael R.
通讯作者：
Ariza, Rafael R.
DOI：
10.1073/pnas.1810582115
发表时间：
2018-12-26
影响因子：
11.1
作者：
Bourbousse, Clara;Vegesna, Neeraja;Law, Julie A.
通讯作者：
Law, Julie A.