A Non-Invasive Method for Generating the Cyclic Loading-Induced Intra-Articular Cartilage Lesion Model of the Rat Knee.
A Non-Invasive Method for Generating the Cyclic Loading-Induced Intra-Articular Cartilage Lesion Model of the Rat Knee.
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DOI：
10.3791/62660
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发表时间：
2021-07-05
期刊：
影响因子：
--
通讯作者：
Ito A
中科院分区：
文献类型：
--
作者：
Ji X;Nakahata A;Zhao Z;Kuroki H;Aoyama T;Ito A
原发性骨关节炎（OA）的病理生理机制尚不清楚。然而，在相对年轻的年龄组中，OA的特定亚分类可能与关节软骨损伤和韧带撕脱的历史有关。膝关节骨性关节炎的外科动物模型在了解创伤后骨性关节炎的发病和进展以及帮助开发这种疾病的新疗法方面发挥着重要作用。然而，非手术模型最近被认为可以避免可能影响干预评估的创伤性炎症。在本研究中，建立了体内循环压缩载荷诱导的关节内软骨损伤大鼠模型，使研究人员能够(1)确定可能引起局灶性软骨损伤的负荷的最佳大小、速度和持续时间；(2)评估创伤后软骨细胞活力的时空病理变化；(3)评估参与关节压缩载荷适应和修复机制的破坏性或保护性分子的组织学表达。本报告描述了在大鼠模型中这种新型软骨病变的实验方案。在此，我们建立了大鼠膝关节关节内软骨损伤模型，该模型通过20 N以上60次循环挤压，导致大鼠股骨髁软骨损伤。
The pathophysiology of primary osteoarthritis (OA) remains unclear. However, a specific subclassification of OA in relatively younger age groups is likely correlated with a history of articular cartilage damage and ligament avulsion. Surgical animal models of OA of the knee play an important role in understanding the onset and progression of post-traumatic OA and aid in the development of novel therapies for this disease. However, non-surgical models have been recently considered to avoid traumatic inflammation that could affect the evaluation of the intervention. In this study, an intra-articular cartilage lesion rat model induced by in vivo cyclic compressive loading was developed, which allowed researchers to (1) determine the optimal magnitude, speed, and duration of load that could cause focal cartilage damage; (2) assess post-traumatic spatiotemporal pathological changes in chondrocyte vitality; and (3) evaluate the histological expression of destructive or protective molecules that are involved in the adaptation and repair mechanisms against joint compressive loads. This report describes the experimental protocol for this novel cartilage lesion in a rat model. Here, we present the cyclic loading-induced intra-articular cartilage lesion model of the rat knee, generated by 60 cyclic compressions over 20 N, resulting in damage to the femoral condylar cartilage in rats.
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影响因子：
7
作者：
Poulet, B.;de Souza, R.;Kent, A. V.;Saxon, L.;Barker, O.;Wilson, A.;Chang, Y. -M.;Cake, M.;Pitsillides, A. A.
通讯作者：
Pitsillides, A. A.
小鼠关节组织对非侵入性膝关节损伤的早期反应提示了治疗目标
影响因子：
13.3
作者：
Wu, P.;Holguin, N.;Silva, M. J.;Fu, M.;Liao, W.;Sandell, L. J.
通讯作者：
Sandell, L. J.
影响因子：
7
作者：
Adebayo OO;Ko FC;Wan PT;Goldring SR;Goldring MB;Wright TM;van der Meulen MCH
通讯作者：
van der Meulen MCH
影响因子：
--
作者：
Poulet, Blandine;Hamilton, Richard W.;Pitsillides, Andrew A.
通讯作者：
Pitsillides, Andrew A.
影响因子：
4.1
作者：
De Souza, RL;Matsuura, M;Pitsillides, AA
通讯作者：
Pitsillides, AA