Intermittent applied mechanical loading induces subchondral bone thickening that may be intensified locally by contiguous articular cartilage lesions.
Intermittent applied mechanical loading induces subchondral bone thickening that may be intensified locally by contiguous articular cartilage lesions.
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DOI：
10.1016/j.joca.2015.01.012
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发表时间：
2015-06
影响因子：
7
通讯作者：
Pitsillides, A. A.
中科院分区：
文献类型：
--
作者：
Poulet, B.;de Souza, R.;Kent, A. V.;Saxon, L.;Barker, O.;Wilson, A.;Chang, Y. -M.;Cake, M.;Pitsillides, A. A.
软骨下骨（SCB）的变化和与关节软骨（AC）的串扰与骨关节炎（OA）有关。本研究使用微型计算机断层扫描（micro-CT）：（1）检查非侵入性负荷小鼠模型中SCB结构的变化，其中选择性地在股骨外侧诱导局灶性AC病变，（2）确定对侧膝关节的任何修改，与步态变化相关，这可能使该肢体作为内部对照的使用复杂化。CBA小鼠右膝关节负重：习惯性使用2周1次（n = 7），连续使用2周（n = 8），连续使用5周（n = 5）。对左（对侧）和右（负载）膝关节进行显微CT扫描，并分析SCB和骨小梁。还进行了步态分析。这些分析显示，负荷5周的关节外侧间室SCB厚度显著增加，在股骨外侧最明显;对侧非负荷膝关节也显示出一过性SCB增厚（负荷一次并重复）。5周负荷后，外侧间室和对侧膝关节所有关节间室的骺骨小梁BV/TV和骨小梁厚度也增加。步态分析表明，从第一次负荷事件后第二周起，施加负荷仅影响所有组小鼠对侧后肢的步态。这些数据表明机械创伤后SCB增厚和AC病变之间存在空间联系，以及在此类OA模型中使用对侧关节作为对照以及可能在OA诊断中使用对侧关节的明显局限性。
Changes in subchondral bone (SCB) and cross-talk with articular cartilage (AC) have been linked to osteoarthritis (OA). Using micro-computed tomography (micro-CT) this study: (1) examines changes in SCB architecture in a non-invasive loading mouse model in which focal AC lesions are induced selectively in the lateral femur, and (2) determines any modifications in the contralateral knee, linked to changes in gait, which might complicate use of this limb as an internal control. Right knee joints of CBA mice were loaded: once with 2weeks of habitual use (n = 7), for 2weeks (n = 8) or for 5weeks (n = 5). Both left (contralateral) and right (loaded) knees were micro-CT scanned and the SCB and trabecular bone analysed. Gait analysis was also performed. These analyses showed a significant increase in SCB thickness in the lateral compartments in joints loaded for 5weeks, which was most marked in the lateral femur; the contralateral non-loaded knee also showed transient SCB thickening (loaded once and repetitively). Epiphyseal trabecular bone BV/TV and trabecular thickness were also increased in the lateral compartments after 5 weeks of loading, and in all joint compartments in the contralateral knee. Gait analysis showed that applied loading only affected gait in the contralateral himd-limb in all groups of mice from the second week after the first loading episode. These data indicate a spatial link between SCB thickening and AC lesions following mechanical trauma, and the clear limitations associated with the use of contralateral joints as controls in such OA models, and perhaps in OA diagnosis.
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影响因子：
7
作者：
Pastoureau, P;Leduc, S;De Ceuninck, F
通讯作者：
De Ceuninck, F
影响因子：
7
作者：
Säämänen, AMK;Salminen, HJ;Metsäranta, MPH
通讯作者：
Metsäranta, MPH
影响因子：
7
作者：
Botter, S. M.;Glasson, S. S.;van Osch, G. J. V. M.
通讯作者：
van Osch, G. J. V. M.
DOI：
10.1016/j.biocel.2004.06.016
发表时间：
2005-01-01
影响因子：
4
作者：
Anderson-MacKenzie, JM;Quasnichka, HL;Bailey, AJ
通讯作者：
Bailey, AJ
影响因子：
4.2
作者：
Li, J;Burr, DB;Turner, CH
通讯作者：
Turner, CH